SVCT

94122105, human breast, Epithelial

• Sinônimo(s): SCTV, SCVT
• Código UNSPSC: 41106514

Propriedades

• product name: SVCT, 94122105, from human breast
• fonte biológica: human breast
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Epithelial
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice

Descrição

Origem de linhagem celular

Human breast epithelium, SV40 transformed

Descrição de linhagem celular

SVCT has been established from human breast epithelial cells being transfected with SV40. The cell line shows an abnormal karyotype and was not tumourigenic in athymic male nude mice. SVCT have been found positive for the expression of keratin 7, 8 and 18 but negative for keratin 19. Epithelial cells, dome formation when confluent.

Perfil do DNA

Not specified

Meio de cultura

DMEM + 2 mM Glutamine + 5μg/ml Hydrocortisone + 10μg/ml Insulin + 10% Fetal Bovine Serum (FBS).

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:2 to 1:4, i.e., seeding at 2-4x10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C.

Outras notas

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Exoneração de responsabilidade

This cell line has special release conditions: All customers are required to complete a Cell line Release Authorisation form.