HAR, G human cell line

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human blood
• embalagem: tube of 5 μg 94082249-DNA-5UG; pkg of vial of cells 94082249-1VL
• etnia: Not specified
• sexo: not specified
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblastoid
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice

Descrição

Descrição geral

Consanguineous: No

Homozygous: No

Descrição de linhagem celular

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Perfil do DNA

HLA DRB1: 0301 07
HLA DRB3: 101
HLA DRB4: 101
HLA DQAP: 2.1 4.1
HLA DQA1: 0501 0201
HLA DQB1: 201
HLA DPB1: 0401 0402

Perfil sorológico

HLA A: 1 32
HLA C: 7
HLA B: 8 14
Bw4/bw6: 6

Meio de cultura

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Rotina de subcultura

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Outras notas

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.