EAV, AC human cell line

94082251, IHW Number: 9151

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human blood
• etnia: Not specified
• sexo: male
• modo de crescimento: Suspension
• cariótipo: Not specified
• morfologia: Lymphoblastoid
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice

Descrição

Descrição geral

Consanguineous: No

Homozygous: No

Descrição de linhagem celular

This cell line is an Epstein-Barr Virus (EBV) transformed lymphoblastoid line and is part of the Human Leukocyte Antigen (HLA) Typed Collection maintained by the European Collection of Cell Cultures (ECACC). HLA-Typing Data is available for this cell line.

Perfil do DNA

A: 02 31
B: 50 37
HLA DRB1: 07 1001
HLA DQAP: 1.3 2.1
HLA DQA1: 0104 0201
HLA DQB1: 0201 0501
HLA DPB1: 0201 0301

Perfil sorológico

HLA A: 2 19
HLA A ief: 2.1 19.1
HLA B: 21 37
HLA B ief:

Meio de cultura

RPMI 1640 (R0883) + 2mM Glutamine (G7513) + 20% Foetal Bovine Serum (FBS) (F2442). Once a growing culture is established the FBS concentration can be reduced to 10%.

Rotina de subcultura

Maintain cultures between 3x10⁵ - 2x10⁶ cells/ml, 5% CO2, 37 °C. Upon resuscitation remove cryoprotectant - centrifuge at 300 x g for 5 minutes and re-suspend the cell pellet in fresh culture medium.

Outras notas

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Exoneração de responsabilidade

This cell line has restrictions and can only be supplied to academic and not-for-profit organisations. Nucleic acids can be supplied to all researchers including those from commercial organisations.