CCRF-HSB-2

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

• Código UNSPSC: 41106514

Propriedades

• fonte biológica: human blood
• embalagem: tube of 5 μg 85112801-DNA-5UG; pkg of vial of cells 85112801-1VL
• modo de crescimento: Suspension
• cariótipo: 2n = 46
• morfologia: Lymphoblast
• produtos: Not specified
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• doença(s) relevante(s): cancer
• Condições de expedição: dry ice
• temperatura de armazenamento: −196°C

Descrição

Origem de linhagem celular

Human Caucasian acute lymphoblastic leukaemia

Descrição de linhagem celular

A T lymphoblastoid line obtained from the peripheral blood of an 11 year old Caucasian male with acute lymphoblastoid leukaemia which has been passaged eight times through new-born Syrian Hamsters. These may be grown to a high density in a spinner type suspension culture.

Perfil do DNA

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,12
D13S317: 10,12
D16S539: 9,13
D5S818: 11,12
D7S820: 11,12
THO1: 9,10
TPOX: 8
vWA: 18,19

Meio de cultura

IDMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Rotina de subcultura

Maintain cultures between 5-8x100,000 cells/ml; 5% CO₂; 37°C. Maximum cell density 2-3x1,000,000 cells/ml.

Outras notas

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