h1RPE7

9061602, human eye, Polygonal

• Código UNSPSC: 41106514

Propriedades

• Nome do produto: h1RPE7, 09061602, from human eye
• fonte biológica: human eye
• modo de crescimento: Adherent
• cariótipo: Not specified
• morfologia: Polygonal
• produtos: Cytokeratins 5 and 8, junctional protein zonula occludens (ZO-1)
• receptores: Not specified
• técnica(s): cell culture | mammalian: suitable
• Condições de expedição: dry ice

Descrição

Origem de linhagem celular

An immortalized retinal pigment epithelial cell line

Descrição de linhagem celular

The h1RPE7 cell line was derived from primary human retinal pigment epithelial cells obtained from a 50-year-old female Caucasian donor. The primary culture was transfected with a construct encoding SV40 large T antigen that was deleted for the small t antigen along with puromycin resistance gene.

Perfil do DNA

STR-PCR Data:
Amelogenin: X
CSF1PO: 10,13
D13S317: 12
D16S539: 12
D5S818: 9,13
D7S820: 9,10
THO1: 7,9.3
TPOX: 8
vWA: 16,19

Meio de cultura

Hams F10 + 20% Heat-inactivated FCS + 2 mM Glutamine + selection antibiotic 1μg/ml puromycin.

Rotina de subcultura

Split sub-confluent cultures (70-80%) 1:4 to 1:10 , i.e., seeding at 3 x 10,000 cells/cm² using 0.05% trypsin or trypsin/EDTA; 5% CO₂; 37 °C. Population doubling approx 24hrs.

Outras notas

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Exoneração de responsabilidade

This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.