The activity of this enzyme is established using a 0.02 M Tris buffer with a starting pH of 8.3 at 0°C. One unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. However, the optimum pH and temperature is highly dependent on the specific application. There are a number of publications regarding the working parameters for this material. Please see the link below to review an example:
https://pubmed.ncbi.nlm.nih.gov/10701452/#:~:text=It%20was%20determined%20that%20the,61706%20Dalton%20by%20gel%20filtration.
C2624
Anidrase carbônica
≥95% (SDS-PAGE), specific activity ≥3,500 W-A units/mg protein, lyophilized powder
Sinônimo(s):
Carbonato desidratase, Carbonato hidrolase
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R$ 2.267,00
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100 MG
R$ 2.267,00
500 MG
R$ 8.544,00
About This Item
Produtos recomendados
fonte biológica
bovine erythrocytes
Nível de qualidade
Ensaio
≥75% protein basis (biuret)
≥95% (SDS-PAGE)
Formulário
lyophilized powder
atividade específica
≥3,500 W-A units/mg protein
solubilidade
deionized water: >10 mg/mL
temperatura de armazenamento
2-8°C
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Aplicação
Ações bioquímicas/fisiológicas
Carbonic Anhydrase is a zinc-containing enzyme that catalyzes the reversible conversion of carbon dioxide to bicarbonate. One of its main physiological roles is to maintain the acid-base balance in blood and other tissues. Lack of carbonic anhydrase results in carbonic anhydrase type II deficiency syndrome, which is an autosomal recessive disease that causes osteopetrosis, renal tubular acidosis and brain calcifications [3].
Definição da unidade
One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0°C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)
Palavra indicadora
Danger
Frases de perigo
Declarações de precaução
Classificações de perigo
Resp. Sens. 1
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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Carbonic anhydrase type II deficiency syndrome is an uncommon autosomal recessive disease with cardinal features including osteopetrosis, renal tubular acidosis and brain calcifications. We describe the neurological, neuro-ophthalmological and neuroradiological features of 23 individuals (10 males, 13 females; ages at
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What is recommand usage temperature and pH range?
1 answer-
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What is the recommended storage conditions for "Carbonic Anhydrase, from bovine erythrocytes?
1 answer-
The recommended storage conditions for Solution of Carbonic Anhydrase, from bovine erythrocytes may be frozen at –20 °C for future use. It is advised to avoid repeated freezing and thawing. Stock solutions may be dispensed into working aliquots, frozen, and then discarded after 2–3 uses.
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What is the isozyme I or isozyme II of Carbonic Anhydrase from bovine erythrocytes (C2624 Carbonic Anhydrase)?
1 answer-
The product C2624, Carbonic Anhydrase from bovine erythrocytes, consists mainly of CA II. However, there are also small levels of CA I and CA IV present.
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Is it possible to obtain information about the type of Carbonic Anhydrase (I or II) included in item C2624-carbonic anhydrase?
1 answer-
Product C2624, Carbonic Anhydrase from bovine erythrocytes, with a purity of ≥95% (SDS-PAGE), consists predominantly of CA II, with small levels of CA I and CA IV present.
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