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C2522

Sigma-Aldrich

Carbonic Anhydrase Isozyme II from bovine erythrocytes

lyophilized powder, ≥3,000 W-A units/mg protein

Sinônimo(s):

Carbonate Dehydratase, Carbonate Hydrolyase, Carbonic Anhydrase II

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About This Item

Número CAS:
9001-03-0
Número da licença da enzima:
4.2.1.1 (BRENDA, IUBMB)
Número CE:
232-576-6
Número MDL:
MFCD00081467
Código UNSPSC:
12352204
NACRES:
NA.54

fonte biológica

bovine erythrocytes

Nível de qualidade

300

Formulário

lyophilized powder

atividade específica

≥3,000 W-A units/mg protein

peso molecular

30 kDa

pI 

~5.9

temperatura de armazenamento

−20°C

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Aplicação

Carbonic anhydrase from sigma has been used for the analysis of thermodynamic stability of the enzyme by measuring the binding affinity and stoichiometry. The enzyme has also been used to generate CD4+ T cell lines specific for carbonic anhydrase during the study of autoimmune pancreatitis in rat.

Ações bioquímicas/fisiológicas

Carbonic anhydrase is a zinc metalloenzyme that has a molecular weight of approximately 30,000 Da. The enzyme catalyzes the hydration of carbon dioxide to carbonic acid. It is involved in many physiological and pathological processes such as pH and CO2 homeostasis, transport of bicarbonate and CO2, biosynthetic reactions, bone resorption, calcification, and tumorigenicity. Therefore, inhibitors that block this enzyme can have therapeutic implications in glaucoma, epilepsy and Parkinson′s disease.

Definição da unidade

One Wilbur-Anderson (W-A) unit will cause the pH of a 0.02 M Trizma buffer to drop from 8.3 to 6.3 per min at 0 °C. (One W-A unit is essentially equivalent to one Roughton-Booth unit.)

Inibidor

Nº do produto
Descrição
Preços

Pictogramas

Health hazard
GHS08

Palavra indicadora

Danger

Frases de perigo

H334

Declarações de precaução

P261 - P284 - P501

Classificações de perigo

Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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Certificados de análise (COA)

Lot/Batch Number
0000308437
0000308440
0000308437
0000308440
SLCQ8559

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Protocolos

Enzymatic Assay of Carbonic Anhydrase for Wilbur-Anderson Units (EC 4.2.1.1)

Objective: To standardize a procedure for the enzymatic assay of Carbonic Anhydrase (EC 4.2.1.1) for Wilbur-Anderson Units.

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