A4268

α-Amylase from porcine pancreas

Type I-A, PMSF treated, saline suspension, 700-1400 units/mg protein (E1%/280)

• Sinônimo(s): β-N-acetylglucosaminidase porcine placenta, PPA, al1,4 glucan-4-glucanohydrolase,, porcine pancreas α-amylase
• Número da licença da enzima: 3.2.1.1 (BRENDA, IUBMB)
• Número MDL: MFCD00081319
• Código UNSPSC: 12352204
• eCl@ss: 32160410
• NACRES: NA.54

Propriedades

• fonte biológica: Porcine pancreas
• tipo: Type I-A
• Formulário: saline suspension
• atividade específica: 700-1400 units/mg protein (E1%/280)
• peso molecular: 51-54 kDa
• características do produto alternativo mais ecológico: Waste Prevention; Design for Energy Efficiency; Learn more about the Principles of Green Chemistry.
• sustainability: Greener Alternative Product
• técnica(s): activity assay: suitable
• adequação: suitable for hydrolysis, synthesis of oligosaccharides and polysaccharides, and sugar modification
• aplicação(ões): diagnostic assay manufacturing
• categoria alternativa mais ecológica: , Enabling
• temperatura de armazenamento: 2-8°C

Descrição

Descrição geral

Molecular mass: 51-54 kDa.
α-Amylase isolated from porcine pancreas is a glycoprotein. It is a single polypeptide chain of ~475 residues containing two SH groups and four disulfide bridges and a tightly bound Ca²⁺ necessary for stability. Chloride ions are necessary for activity and stability. The pH range for activity is 5.5 to 8.0, with the pH optimum at 7.

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Aplicação

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. α-Amylase, from Sigma, has been used in various plant studies, such as metabolism studies in Arabidopsis [1].

Ações bioquímicas/fisiológicas

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. α -Amylase, from porcine pancreas, is a glycoprotein that consists of a single polypeptide chain of approximately 475 residues containing 2 SH groups and four disulfide bridges and a tightly bound Ca²⁺ necessary for stability.

Definição da unidade

One unit will liberate 1.0 mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

forma física

Suspension in 2.9 M NaCl solution containing 3 mM CaCl_2.

Nota de preparo

2× crystallized

Outras notas

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P342 + P311
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable
• Equipamento de proteção individual: dust mask type N95 (US), Eyeshields, Faceshields, Gloves