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Documentos Principais

A9857

Sigma-Aldrich

αα-Amilase

greener alternative

≥150 units/mg protein (biuret)

Sinônimo(s):

1,4-α-D-Glicano glicano-hidrolase

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About This Item

Número da licença da enzima:
Número CE:
Número MDL:
Código UNSPSC:
12352204
eCl@ss:
32160410
NACRES:
NA.54
Preço e disponibilidade não estão disponíveis no momento.

Formulário

powder

atividade específica

≥150 units/mg protein (biuret)

características do produto alternativo mais ecológico

Waste Prevention
Design for Energy Efficiency
Learn more about the Principles of Green Chemistry.

sustainability

Greener Alternative Product

categoria alternativa mais ecológica

temperatura de armazenamento

−20°C

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Descrição geral

We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for energy efficiency and waste prevention when used in starch ethanol research. For more information see the article in biofiles.

Aplicação

α-Amylase is used to hydrolyze α bonds of α-linked polysaccharides, such as starch and glycogen. α-Amylase has been used in various plant studies, such as metabolism studies in Arabidopsis [1]. Amylases from Aspergillus oryzae are commonly used as baking additives to prevent staling in the baking industry, clarify haze from fruit juices and alcoholic beverages, and to produce glucose and maltose syrup products [2].

Ações bioquímicas/fisiológicas

α-Amylase hydrolyzes the α-(1,4) glucan linkages in polysaccharides of three or more α-(1,4) linked D-glucose units. Natural substrates such as starch and glycogen are broken down into glucose and maltose. The International Union of Immunological Societies (IUIS) has classified α-amylase from Aspergillus oryzae (Asp o II) as an occupational allergen that can cause respiratory symptoms [2].

Qualidade

Crude

Definição da unidade

One unit will liberate 1.0mg of maltose from starch in 3 min at pH 6.9 at 20 °C.

forma física

powder

Pictogramas

Health hazardExclamation mark

Palavra indicadora

Danger

Frases de perigo

Classificações de perigo

Acute Tox. 4 Oral - Resp. Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

dust mask type N95 (US), Eyeshields, Faceshields, Gloves


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Certificados de análise (COA)

Lot/Batch Number

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X Baur et al.
Clinical and experimental allergy : journal of the British Society for Allergy and Clinical Immunology, 24(5), 465-470 (1994-05-01)
The commercially available alpha-amylase from Aspergillus oryzae which is widely used as a baking additive was compared with a highly purified enzyme preparation. We used enzyme allergosorbent test (EAST), EAST inhibition, sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), isoelectric focussing, immunoblotting
Sean McClure
Journal of AOAC International, 103(4), 1060-1072 (2020-11-27)
A multi-laboratory study was completed for AOAC First Action Method 2015.14. Ten laboratories from eight countries participated. Each laboratory analyzed (in duplicate) a placebo and 14 fortified nutritionals. Product matrices analyzed included milk, soy, partially hydrolyzed milk, partially hydrolyzed soy
Marit Navis et al.
Nutrients, 12(11) (2020-11-08)
Human milk is the optimal diet for infant development, but infant milk formula (IMF) must be available as an alternative. To develop high-quality IMF, bovine milk processing is required to ensure microbial safety and to obtain a protein composition that
Jesus Valcarcel et al.
Polymers, 14(1) (2022-01-12)
Styela clava is an edible sea squirt farmed in Korea that has gradually invaded other seas, negatively impacting the ecology and economy of coastal areas. Extracts from S. clava have shown wide bioactivities, and ascidians have the unique capability among
Fengyuan Liu et al.
Food chemistry, 350, 129259-129259 (2021-02-24)
Cereals and legumes are rich in folate. However, due to the instability of folate, processing and digestion can induce significant folate loss. In this paper, folate bioaccessibility of faba bean, oat, rye and wheat flours and pastes was studied using

Protocolos

Follow our procedure for the determination of alpha-Amylase activity. This enzymatic assay of a-Amylase guides you through the entire process and necessary calculations.

Questions

  1. Please specify quantity for 250KU product no A9857

    1 answer
    1. This product is sold by activity. The minimum activity specification for this material is 150 units per milligram. The weight of this product can be determined using the activity value reported on the lot-specific Certificate of Analysis(COA). For example, the sample COA has an activity value of 280 units/mg. The amount of powder in the 250,000-unit package will be 892.85mg.

      Please navigate to the ‘DOCUMENTATION’ section of the Product Detail Page to access a sample or lot-specific Certificate:
      https://www.sigmaaldrich.com/product/sigma/a9857#product-documentation

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