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Sigma-Aldrich

Estreptavidina

affinity purified, lyophilized from 10 mM potassium phosphate, ≥13 U/mg protein

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About This Item

Número CAS:
9013-20-1
Número MDL:
MFCD00082035
Código UNSPSC:
12352202
NACRES:
NA.32

forma

powder

Nível de qualidade

200

qualidade

affinity purified
lyophilized from 10 mM potassium phosphate

atividade específica

≥13 U/mg protein

peso molecular

Mr ~60000

solubilidade

H2O: 1 mg/mL, clear to hazy, colorless to faintly yellow

temperatura de armazenamento

−20°C

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Descrição geral

Streptavidin is a crystalline protein synthesized by Streptomycetes.

Aplicação

Streptavidin from Streptomyces avidinii has been used:
  • in the pre-functionalization of self-propelled catalytic micromotors
  • in the fabrication and modification of hydroxyapatite-chitosan (HA-CTS) nanofilm-coated substrates
  • as an analyte to bind biomolecules to the gold-inverted polymer solar cells (Au-IPSC)

Ações bioquímicas/fisiológicas

Streptavidin is a biotin binding protein and has the ability to bind four molecules of biotin. The biotin binding pocket of streptavidin helps to interact with biotin. It can be used to enhance protein binding and multimerization.

Embalagem

Bottomless glass bottle. Contents are inside inserted fused cone.
The sales quantitites 1 mg and 5 mg

Definição da unidade

1 U corresponds to the amount of protein which binds 1μg (+)-biotin at pH 7.5

Nota de análise

Binding capability: Streptavidin binds one molecule of biotin per subunit. Comparison of biotin binding and absorbance measurements indicates that ≤5% binding sites are occupied.

Outras notas

Review: Application of the avidin-biotin technique in microbiology; Protein isolated from the bacterium Streptomyces avidinii, having a high affinity for biotin; Avidin-Biotin technology

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)

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