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11089153001

Roche

Streptavidin-POD conjugate

liquid, pkg of 1 mL

Sinônimo(s):

streptavidin

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About This Item

Código UNSPSC:
12352200
Preço e disponibilidade não estão disponíveis no momento.

Formulário

liquid

Nível de qualidade

embalagem

pkg of 1 mL

fabricante/nome comercial

Roche

temperatura de armazenamento

-10 to -25°C

Descrição geral

Streptavidin is covalently coupled to horseradish peroxidase. The conjugate is used for detection of biotin-labelled substances.

Aplicação

Streptavidin-POD has been used for:
  • ELISA[1]
  • In situ hybridization[2]
  • Western blot[3]
It is also used for immunohistochemistry.

Definição da unidade

One unit Streptavidin-POD, corresponding to one unit peroxidase, will oxidize 1 μmol of guaiacol tetramer in one minute at +25 °C and pH 7.0.

Unit Conversion: 1 U (+25 °C; guaiacol as substrate; pH7.0) corresponds to 1.24 U (+25 °C; 1.7 mM ABTS as substrate; pH= 6.0) or to 4.0 U (+25 °C; 8.7 mM ABTS as substrate; pH = 5.0).

forma física

Liquid, stabilized

Nota de preparo

Working concentration: 5 - 500 mU/ml (i.e., stock solution can be diluted).Working concentration of conjugate depends on application and substrate. The following concentrations should be taken as a guideline:
  • ELISA: 50 to 200 mU/ml
  • Immunohistocytochemistry: 250 to 1000 mU/ml
  • Western blot: 200 to 500 mU/ml

Outras notas

For life science research only. Not for use in diagnostic procedures.

Pictogramas

Exclamation mark

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Skin Sens. 1

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash


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Husheem Michael et al.
Frontiers in immunology, 11, 196-196 (2020-03-03)
Human rotavirus (HRV) is a leading cause of morbidity and mortality in children, especially in developing countries. Malnutrition is prevalent in these countries, which may contribute to the decreased oral vaccine efficacy, posing a concern for global health. Neonatal gnotobiotic
Angelika Hammerer-Lercher et al.
Clinical chemistry, 54(5), 858-865 (2008-03-15)
The specific forms of pro-B-type natriuretic peptide (proBNP) that occur in human blood are not yet clear. We demonstrated the presence of several proBNP forms in human plasma with a new affinity chromatography method that can be used in combination
BAP1 and PTEN mutations shape the immunological landscape of clear cell renal cell carcinoma and reveal the intertumoral heterogeneity of T cell suppression: a proof-of-concept study.
Friedhoff, et al.
Cancer Immunology, Immunotherapy, 72, 1603-1618 (2023)
Fernanda V Castro et al.
Cancer immunology, immunotherapy : CII, 61(7), 1005-1018 (2011-12-01)
Effective vaccination against tumour-associated antigens (TAA) such as the 5T4 oncofoetal glycoprotein may be limited by the nature of the T cell repertoire and the influence of immunomodulatory factors in particular T regulatory cells (Treg). Here, we identified mouse 5T4-specific

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