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Sigma-Aldrich

Anti-Rabbit IgG−Abberior® STAR RED antibody produced in goat

for STED application

Sinônimo(s):

Abberior® STAR RED-Anti-Rabbit IgG antibody produced in goat

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About This Item

Código UNSPSC:
12352200
NACRES:
NA.32

fonte biológica

goat

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

secondary antibodies

clone

polyclonal

forma

buffered aqueous solution

reatividade de espécies

rabbit

concentração

~1 mg/mL

fluorescência

λex 638 nm; λem 655 nm in PBS, pH 7.4

temperatura de armazenamento

−20°C

Descrição geral

Abberior STAR RED is a well-known red dye, named also KK114 in literature. It was developed for STED and confocal imaging in the red spectral region. It shows an exceptional brightness and low background, delivering stunning STED images. The dye works extremely well with Abberior Instruments STEDYCON and Expert Line microscopes and Leica STED microscopes. Abberior STAR RED can substitute dyes like Atto 647N, Alexa Fluor 647, or Cy®5. It can be excited with lasers at wavelength between 630 - 650 nm. For STED, a depletion wavelength around 750 - 800 nm is recommended. Abberior STAR RED is the ideal partner for Abberior STAR 580 to obtain optimal 2 Color STED results. Best results are obtained with freshly prepared samples.


Photophysical properties (carboxylic acid):
Absorption Maximum, λex [nm]: 638 (PBS pH 7.4), 638 (H2O), 634 (MeOH)
Extinction Coefficient, εmax [M-1cm-1]: 120 000 (PBS pH 7.4), 125 000 (H2O), 115 000 (MeOH)
Correction Factor, CF260 = ε260max: 0,16 (PBS pH 7.4)
Correction Factor, CF280 = ε280max: 0,32 (PBS pH 7.4)
Fluorescence Maximum, λem [nm]: 655 (PBS pH 7.4), 655 (H2O), 654 (MeOH)
Recommended STED Wavelength, λ [nm]: 750-800
Fluorescence Quantum Yield, λ: 0,90 (PBS pH 7.4)
Fluorescence Lifetime, τ [ns]: 3,4 (PBS pH 7.4)

Características e benefícios

  • Unmatched, background free STED imaging cont
  • Verified in Abberior Instruments and Leica STED microscopes

Adequação

Designed and tested for fluorescent super-resolution microscopy

Nota de análise

May contain significant amounts of BSA as stabilizer
unconjugated dye ≤5% of total fluorescence

Informações legais

ALEXA FLUOR is a trademark of Life Technologies
Atto is a trademark of Atto-Tec GmbH
Cy is a registered trademark of Cytiva
abberior is a registered trademark of Abberior GmbH

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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