Modified Giemsa stain means when the pH of the Giemsa stain is changed from the usual 6.8 to 9.0.[1]
Aplicação
Modified Giemsa stain may be used for the identification of each human chromosome by differentials staining.[1]
Outras notas
Giemsa stain, modified, 0.4%, w/v, in buffered methanol solution, pH 6.8. Also contains stabilizers.
Princípio
When blood films are stained using Giemsa Stain, the nucleus and cytoplasm of white blood cells take on characteristic blue or pink coloration. The use of purified eosin and thiazine dyes minimizes lot-to-lot variation.
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Differential staining of human chromosomes can be obtained when the pH of Giemsa stain is changed to 9.0 from the usual 6.8. Such staining permits identification of all homolog pairs and distinct regions within chromosome arms. In most instances, the
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