Giemsa stain, as defined by the Biological Stain Commission, consists of a polychrome methylene blue and eosin y mixture. When methylene blue undergoes the polychroming process, various homologs of methylene blue are expected to be present. Azure A, Azure B, Azure C, and other homologs may be present. The percentages of the various homologs can and will vary. This product is not tested to determine the identity or concentration of these homologs.
G5637
Giemsa stain
certified by the Biological Stain Commission, powder or crystals
Sinônimo(s):
Azure mixture sicc. Giemsa stain
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5 G
R$ 1.074,00
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R$ 3.721,00
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5 G
R$ 1.074,00
25 G
R$ 3.721,00
About This Item
Fórmula empírica (Notação de Hill):
C14H14ClN3S
Número CAS:
Peso molecular:
291.80
Número MDL:
Código UNSPSC:
12171500
ID de substância PubChem:
NACRES:
NA.47
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Nome do produto
Giemsa stain, certified by the Biological Stain Commission
grau
certified by the Biological Stain Commission
Nível de qualidade
Formulário
powder or crystals
técnica(s)
microbe id | staining: suitable
cor
dark green to very dark green
pf
300 °C (lit.)
solubilidade
ethanol: 1 mg/mL, blue to very deep blue
Absorção
≥0.6 at 647-653 nm in methanol at 0.005 g/L
aplicação(ões)
diagnostic assay manufacturing
hematology
histology
temperatura de armazenamento
room temp
cadeia de caracteres SMILES
[Cl-].CN(C)c1ccc2N=C3C=CC(=[NH2+])C=C3Sc2c1
InChI
1S/C14H13N3S.ClH/c1-17(2)10-4-6-12-14(8-10)18-13-7-9(15)3-5-11(13)16-12;/h3-8,15H,1-2H3;1H
chave InChI
NALREUIWICQLPS-UHFFFAOYSA-N
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Descrição geral
Giemsa is a versatile polychromatic stain, which is suitable for staining a diverse range of specimens. It is a buffered thiazine-eosinate solution designed to provide coloration of blood cells. Giemsa’s stain is frequently used for diagnostic purposes in hematology to differentiate the nuclear and cytoplasmic morphology of platelets, RBCs, WBCs, and parasites. It is frequently used in combination with other dye solutions as May-Grunwald’s solution for Pappenheim (MGG) and Wright-Giemsa. The Giemsa stain was adapted to histology due to its unique staining of chromatin, nuclear membranes, and cytoplasmic elements. Giemsa′s staining solution is composed of methylene blue, azure, and eosin.
Aplicação
Giemsa stain has been used for the staining of cells and blood films.[1][2]It is required for the staining of azurophil granules and is an excellent stain for inclusion bodies.[3]Its applications can be seen in hematology for blood and bone marrow specimens, bacteriology, clinical cytology specimens, histological biopsies, and tumor samples.
Adequação
Certified for Giemsa stain for blood films and for the Wolbach′s Giemsa method for paraffin sections.
Outras notas
Nuclei will be varying shades of purple. Cytoplasmic staining will be varying shades of blue to light pink. Fine reddish to lilac granules may be present in cytoplasm of some cell types. Basophils will demonstrate dark blue black granules in the cytoplasm. Eosinophils will demonstrate bright orange granules in the cytoplasm. Red blood cells should be pink to orange
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Equipamento de proteção individual
dust mask type N95 (US), Eyeshields, Gloves
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Hello, could you confirm the dye(s) present in G5367? Is it only the pictured compound (I think Azure A) without methylene blue or eosin? Thank you!
1 answer-
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