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Documentos Principais

12006C

Sigma-Aldrich

Soro bovino fetal

Australia origin, USDA approved, Heat Inactivated, suitable for cell culture

Sinônimo(s):

FBS, FCS, soro, soros

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About This Item

Número MDL:
Código UNSPSC:
12352207
NACRES:
NA.71
fonte biológica:
bovine fetus
origem:
Australia origin
esterilidade:
sterile-filtered
Condições de expedição:
dry ice
Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

bovine fetus

esterilidade

sterile-filtered

linha de produto

FBS Premier

qualidade

USDA approved

composição

Hemoglobin, ≤25 mg/dL

origem

Australia origin

técnica(s)

cell culture | mammalian: suitable

Impurezas

≤10 EU/mL Endotoxin

Condições de expedição

dry ice

temperatura de armazenamento

−20°C

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Descrição geral

FBS remains a popular media supplement because it provides a wide array of functions in cell culture. FBS delivers nutrients, growth and attachment factors and protects cells from oxidative damage and apoptosis by mechanisms that are difficult to reproduce in serum-free media (SFM) systems.

Aplicação

FBS sourced from Australia is used in a broad range of cell culture applications. FBS provides many non-defined growth promoting and survival enhancing factor to cells in culture. Australia carries the best possible rating in relation to Geographical Bovine Spongiform Encephalopathy (BSE) Risk (GBR) as designated by the Scientific Steering Committee on the Geographical Risk of Bovine Spongiform Encephalopathy. The classification for Australia is GBR Level I.

Nota de preparo

Heat inactivated for 30 minutes at 56° C.

Nota de análise

  • Testado quanto à presença de endotoxinas e hemoglobina
  • Tested for the presence of bacteria, virus, and mycoplasma
  • Triple filtered with 0.1 micron membrane under aseptic conditions

Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves


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Certificados de análise (COA)

Lot/Batch Number

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Questions

1–2 of 2 Questions  
  1. Once a bottle of Fetal Bovine Serum (FBS) is thawed, for how long can the bottle be used?

    1 answer
    1. Once Fetal Bovine Serum has been thawed, the bottle can be used in cell culture for up to 7 days for most cell types when stored  refrigerated at 2-8°C.  If the cells are not fastidous (e.g. HeLa or fibroblasts), the thawed and refrigerated serum can be used for up to 30 days.  For some extremely fastidious cell lines, it may be necessary to screen individual lots for acceptable performance.  For uses such as blocking for various immuno procedures, usage will generally be for 30 days and perhaps somewhat longer when stored at 2-8°C.  For other procedures, usage beyond 30 days storage at 2-8°C will need to be determined.

      Helpful?

  2. What is the Department of Transportation shipping information for this product?

    1 answer
    1. Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.

      Helpful?

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