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D0531

Sigma-Aldrich

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride

≥90% purity (HPLC), powder

Sinônimo(s):

DTAF

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About This Item

Fórmula empírica (Notação de Hill):
C23H12Cl2N4O5 · HCl
Número CAS:
Peso molecular:
531.73
Número MDL:
Código UNSPSC:
12171500
ID de substância PubChem:
NACRES:
NA.47

product name

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride, ≥90% (HPLC)

Nível de qualidade

Ensaio

≥90% (HPLC)

forma

powder

cor

yellow to orange

solubilidade

methanol: 20 mg/mL, clear to slightly hazy, yellow to orange

fluorescência

λex 495 nm; λem 516 nm

aplicação(ões)

diagnostic assay manufacturing
hematology
histology

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

Cl.Oc1ccc2c(Oc3cc(O)ccc3C24OC(=O)c5cc(Nc6nc(Cl)nc(Cl)n6)ccc45)c1

InChI

1S/C23H12Cl2N4O5.ClH/c24-21-27-22(25)29-23(28-21)26-10-1-4-13(16(7-10)20(32)33)19-14-5-2-11(30)8-17(14)34-18-9-12(31)3-6-15(18)19;/h1-9,30H,(H,32,33)(H,26,27,28,29);1H

chave InChI

CEKSHCAIHGNWBK-UHFFFAOYSA-N

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Aplicação

5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used for labeling of yeast cells to study the adhesion of yeast cells to the digestive tract of sea bass (Dicentrarchus labrax) larvae and leopard grouper (Mycteroperca rosacea). It has also been used to label bacteria (Vibrio spp.).
5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride has been used to covalently label xanthan gum (XG) to study its gelatin behavior.
A fluorescein derivative which reacts with amino groups and N-terminal amino acids of proteins at neutral pH.

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Synergistic gelation of gelatin B with xanthan gum
Wang CS, et al.
Food Hydrocolloids, 60, 374-383 (2016)
Assessment of fluorescent-labeled bacteria for evaluation of in vivo uptake of bacteria (Vibrio spp.) by crustacean larvae.
Soto-Rodriguez SA, et al.
Journal of Microbiological Methods, 52, 101-114 (2003)
Adrián Pérez-Ramos et al.
Frontiers in microbiology, 9, 1684-1684 (2018-08-10)
Lactic acid bacteria synthesize exopolysaccharides (EPS), which could benefit the host's health as immunomodulators. Furthermore, EPS could protect bacteria against gastrointestinal stress, favoring gut colonization, thus protecting the host against pathogenic infections. Pediococcus parvulus 2.6, produces a 2-substituted (1,3)-β-D-glucan and
Michael S Reid et al.
Carbohydrate polymers, 250, 116943-116943 (2020-10-15)
Fluorescently labeled cellulose nanofibrils (CNFs) were used to evaluate CNF leaching from paper according to standard safety assays for food contact materials. Enzymatically pretreated pulp was first labeled with 5-([4,6-Dichlorotriazin-2-yl]amino)fluorescein hydrochloride (DTAF), followed by homogenization to produce fluorescent CNFs of
A gelation mechanism for gelatin/polysaccharide aqueous mixtures
Wang CS, et al.
Food Hydrocolloids, 79, 462-472 (2018)

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