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46945

Sigma-Aldrich

Isotiocianato de fluoresceína–dextrana

average mol wt 70,000, (FITC:Glucose = 1:250)

Sinônimo(s):

FITC–Dextrana

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100 MG
R$ 1.053,00
500 MG
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About This Item

Número CAS:
Número MDL:
Código UNSPSC:
12352201
NACRES:
NA.32

R$ 1.053,00


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fonte biológica

synthetic

conjugado

FITC conjugate

Formulário

powder

peso molecular

average mol wt 70,000

composição

(FITC:Glucose = 1:250)

Extensão da rotulagem

~0.004 mol/mol FITC glucose

solubilidade

H2O: 50 mg/mL, hazy, orange to very deep orange

fluorescência

λex 492 nm; λem 518 nm in ethanol

aplicação(ões)

cell analysis

temperatura de armazenamento

2-8°C

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Aplicação

Dextrana marcada com isotiocianato de fluoresceína para possível uso em estudos de perfusão em animais.
Fluorescein isothiocyanate–dextran (FITC-Dextran 70) is use as a fluorescent probe to study cell processes such as cell permeability, phagocytosis and endocytosis and to study mechanisms of biomolecular delivery. FITC-Dextran 70 may be used to study the capacity, selectivity and efficiency of encapsulation and release by drug delivery vehicles.

Outras notas

Internalisation of FITC and FITC-dextran by suspension cultured plant cells[1]; In the study of fluid pinocytosis in leukocytes[2]

Pictogramas

Exclamation mark

Palavra indicadora

Warning

Frases de perigo

Classificações de perigo

Eye Irrit. 2 - Skin Irrit. 2 - STOT SE 3

Órgãos-alvo

Respiratory system

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Equipamento de proteção individual

Eyeshields, Gloves, type N95 (US)


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Use of horseradish peroxidase and fluorescent dextrans to study fluid pinocytosis in leukocytes.
J M Oliver et al.
Methods in enzymology, 108, 336-347 (1984-01-01)
L. Cole et al.
Journal of Cell Science, 96, 721-721 (1990)
Misty R Riddle et al.
Developmental biology, 441(2), 285-296 (2018-06-09)
Through the course of evolution, the gastrointestinal (GI) tract has been modified to maximize nutrient absorption, forming specialized segments that are morphologically and functionally distinct. Here we show that the GI tract of the Mexican tetra, Astyanax mexicanus, has distinct
Siegfried Weisenburger et al.
Cell, 177(4), 1050-1066 (2019-04-16)
Calcium imaging using two-photon scanning microscopy has become an essential tool in neuroscience. However, in its typical implementation, the tradeoffs between fields of view, acquisition speeds, and depth restrictions in scattering brain tissue pose severe limitations. Here, using an integrated
Louise Coppin et al.
Cells, 8(8) (2019-08-10)
The efficacy of mesenchymal stem cell infusion is currently tested in numerous clinical trials. However, therapy-induced thrombotic consequences have been reported in several patients. The aim of this study was to optimize protocols for heterologous human adult liver-derived progenitor cell

Questions

1–2 of 2 Questions  
  1. After reconstituting in water, what are the storage conditions?

    1 answer
    1. The stability of this material is solution has not been determined. However, aqueous solutions of 25 mg/mL or more of FITC-Dextran are expected to be stable for 1-2 weeks when stored refrigerated at 2-8°C and protected from light. For long-term storage, aliquot and store stock solutions at ?20°C, protected from light.

      Helpful?

  2. Is this product fixable?

    1 answer
    1. Yes, paraformaldehyde can be used to fix cells. In normal circumstances, formaldehyde is a cross-linking fixative that is used to fix protein and DNA.

      Looking at formaldehyde fixation, the following are applicable:
      Formaldehyde cross-links with the amino group of the N-terminal amino acid residue and the side chains of 12:
      Arginine
      Cysteine
      Histidine
      Lysine
      Depending on the peptide sequence, methylol groups, Schiff-bases, and methylene bridges were form

      Please note that although the term paraformaldehyde is used, paraformaldehyde exists only as a solid. Once the bonds of the polymer are broken, solutions contain only formaldehyde. Any paraformaldehyde in the solution would simply be an undissolved precipitate at the bottom of the solution.

      Helpful?

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