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77619

Supelco

Mistura de fenol – clorofórmio – álcool isoamílico

Phenol – chloroform – isoamyl alcohol mixture

BioUltra, for molecular biology, 125:24:1

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About This Item

Número CAS:
136112-00-0
Número MDL:
MFCD00133763
Código UNSPSC:
12000000
ID de substância PubChem:
329767819
NACRES:
ND.02
grau:
for molecular biology
Preço e disponibilidade não estão disponíveis no momento.

grau

for molecular biology

Nível de qualidade

100

linha de produto

BioUltra

Formulário

liquid

Impurezas

DNases, none detected
 RNases, none detected
 phosphatases, none detected
 proteases, none detected

pH

4-5(H2O-phase, after extraction with H2O, 1:1)

densidade

1.12 g/mL at 20 °C

λ

neat

Absorção UV

λ: 330 nm Amax: 0.2
λ: 405 nm Amax: 0.1
λ: 510 nm Amax: 0.1

temperatura de armazenamento

2-8°C

cadeia de caracteres SMILES

ClC(Cl)Cl.CC(C)CCO.Oc1ccccc1

InChI

1S/C6H6O.C5H12O.CHCl3/c7-6-4-2-1-3-5-6;1-5(2)3-4-6;2-1(3)4/h1-5,7H;5-6H,3-4H2,1-2H3;1H

chave InChI

ZYWFEOZQIUMEGL-UHFFFAOYSA-N

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Aplicação


  • Simultaneous quantification of multiple RNA cargos co-loaded into nanoparticle-based delivery systems: This research explores the application of the Phenol – chloroform – isoamyl alcohol mixture in extracting high-quality RNA for advanced therapeutic formulations, emphasizing its critical role in nanotechnology and pharmaceutical sciences (Lokras et al., 2022).

  • Analysis of Circulating microRNAs and Their Post-Transcriptional Modifications in Cancer Serum by On-Line Solid-Phase Extraction-Capillary Electrophoresis-Mass Spectrometry: Demonstrates the use of the Phenol – chloroform – isoamyl alcohol mixture for precise extraction of microRNAs from biological fluids, contributing to cancer diagnostics and molecular pathology (Pero-Gascon et al., 2018).

  • The application of alkaline lysis and pressure cycling technology in the differential extraction of DNA from sperm and epithelial cells recovered from cotton swabs: Highlights the integration of the Phenol – chloroform – isoamyl alcohol mixture with novel technologies for forensic applications, enhancing DNA extraction processes critical in legal and criminal investigations (Nori and McCord, 2015).

  • Frequency of Epstein-Barr virus DNA sequences in human gliomas: Utilizes the Phenol – chloroform – isoamyl alcohol mixture for DNA extraction in medical research, facilitating the study of viral presence in cancer tissues and its implications for oncology and virology (Fonseca et al., 2015).

  • The critical role of DNA extraction for detection of mycobacteria in tissues: This study underscores the effectiveness of the Phenol – chloroform – isoamyl alcohol mixture in the molecular detection of pathogens, serving as a fundamental technique in microbiological diagnostics and infectious disease research (Radomski et al., 2013).

forma física

mixture of phenol; chloroform; isoamyl alcohol in the ratio of 125:24:1 (v/v/v); saturated with 2 M NaOAc pH 4

Palavra indicadora

Danger

Classificações de perigo

Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 2 - Carc. 2 - Eye Dam. 1 - Muta. 2 - Repr. 2 - Skin Corr. 1B - STOT RE 1 Oral - STOT RE 2

Órgãos-alvo

Liver,Kidney, Nervous system,Kidney,Liver,Skin

Código de classe de armazenamento

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

Classe de risco de água (WGK)

WGK 3

Equipamento de proteção individual

Faceshields, Gloves, Goggles, type ABEK (EN14387) respirator filter

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Certificados de análise (COA)

Lot/Batch Number
BCCL1272
BCCJ9126
BCCD7662
BCBZ6964
BCBS4114V

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Isoamyl alcohol ≥98%, FG

Sigma-Aldrich

W205702

Isoamyl alcohol

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  1. Vancouver, BC
    • Review 1
    • Votes 2
    1 out of 5 stars.

    Did not work as a replacement for P1944 to isolate RNA

    We used to use P1944, but it was back ordered. So we decided to give this product a try.

    We performed RNA extractions from the same culture using either P1944 or 77619. P1944 gave intact ribosomal bands with high concentration, whereas 77619 gave low concentration of RNA with degraded RNA.

    We reached out, received no technical support, but was offered a refund.

    Helpful?

    1. Response from MilliporeSigma:

      Thank you for taking the time to leave a review! We are sorry to hear that your experience did not meet expectations and apologize that no technical support was provided at the time of refund. A member of our Technical Service team will be contacting you shortly in order to troubleshoot this issue and suggest a replacement product.

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