Pular para o conteúdo
Merck
Todas as fotos(5)

Documentos

PFLRKB

Roche

KAPA PROBE FAST

suitable for qPCR, 2 ×, ROX™ Low

Sinônimo(s):

Probe qPCR, qPCR

Faça loginpara ver os preços organizacionais e de contrato
Todas as fotos(5)

About This Item

Código UNSPSC:
41106300
NACRES:
NA.55

Nível de qualidade

100

uso

sufficient for 100 reactions
 sufficient for 1000 reactions
 sufficient for 500 reactions

prazo de validade

≤12 mo.

Características

dNTPs included
hotstart

embalagem

kit of 1 mL (100 x 20 μL rxn; KK4716)
kit of 10 mL (1000 x 20 μL rxn; KK4718)
kit of 5 mL (500 x 20 μL rxn; KK4717)
pkg of

fabricante/nome comercial

Roche

concentração

2 ×

técnica(s)

qPCR: suitable

entrada

purified DNA

compatibilidade

for use with Agilent Mx3000P
for use with Agilent Mx3005P
for use with Agilent Mx4000
for use with Applied Biosystems® 7500
for use with Applied Biosystems® ViiA 7
for use with QuantStudio™

método de detecção

probe-based

temperatura de armazenamento

−20°C

Procurando produtos similares? Visita Guia de comparação de produtos

Descrição geral

KAPA PROBE FAST qPCR Kits provide fast and reproducible results for all probe-based qPCR applications. Kits contain a ready-to-use master mix for highly sensitive and accurate real-time PCR using sequence-specific fluorogenic probe chemistries, including hydrolysis probes (e.g. TaqMan®), FRET probes, and displacement probes (e.g. molecular beacons).

Aplicação

KAPA PROBE FAST qPCR Master Mix (2X) with ROX™ Low is suitable for use in:
  • Genotyping
  • Gene expression analysis
  • Multiplex qPCR
  • SNP genotyping/allelic discrimination
  • Microarray validation
  • Real time(RT)-quantitative polymerase chain reaction (qPCR)/qPCR

Características e benefícios

Accurately discriminate between alleles:
  • Exceptional differentiation of heterozygous and homozygous alleles
  • Discrete clusters in SNP genotyping assays

Achieve excellent reproducibility and efficiency:
  • Fast, high performance five-colored multiplex PCR
  • Similar abundance levels achieved with low copy number
  • Broad dynamic range of up to 10 orders of magnitude

Increase flexibility:
  • Compatible with both standard and fast cycling protocolsRetain accurate quantification

Quick Notes:
  • These kits are compatible with all fluorogenic probebased technologies, including hybridization probes (e.g., FRET), hydrolysis probes (e.g., TaqMan®) and displacement probes (e.g., molecular beacons).
  • Initial denaturation for 20 sec at 95ºC is sufficient for enzyme reactivation; however, optimal denaturation of complex targets may require up to 3 min denaturation.
  • For 2-step cycling, use 30 sec combined annealing/ extension/data acquisition.
  • For 3-step cycling, use 20 sec for primer annealing and 1 sec for extension/data acquisition.
  • Do not exceed 25 μL volumes.

Qualidade

KAPA PROBE FAST qPCR Master Mix (2X) is subjected to stringent quality control tests, is free of contaminating exoand endonuclease activity, and meets strict requirements with respect to DNA contamination levels.

Nota de preparo

ROX/fluorescein reference dyes (depending on kit configuration) are light sensitive. Exposure to direct light for an extended period of time will result in loss of fluorescent signal intensity. Avoid repeated freezing and thawing. Always ensure that the product has been fully thawed and mixed before use.

KAPA PROBE FAST qPCR Master Mix (2X) is stable through 30 freeze-thaw cycles. Ensure that all reagents are stored protected from light at -20°C when not in use. When protected from light, reagents are stable in the dark at 4°C for at least one week, and may be stored at this temperature for short-term use provided that they do not become contaminated with microbes and/or nucleases.

Outras notas

For Research Use Only. Not for use in diagnostic procedures.

Informações legais

Applied Biosystems is a registered trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
QuantStudio is a trademark of IROA Technologies LLC
ROX is a trademark of Applera Corporation or its subsidiaries in the US and/or certain other countries
TaqMan is a registered trademark of Roche Molecular Systems, Inc.

Somente componentes do kit

Nº do produto
Descrição
  • KAPA PROBE FAST qPCR Master Mix (2X) with ROX™ Low
  • KAPA Taq HotStart® DNA Polymerase
  • reaction buffer
  • dNTPs
  • MgCl2 5 nM

Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

does not flash

Ponto de fulgor (°C)

does not flash

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

Já possui este produto?

Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.

Visite a Biblioteca de Documentos

Johan Henriksson et al.
Cell, 176(4), 882-896 (2019-01-15)
T helper type 2 (Th2) cells are important regulators of mammalian adaptive immunity and have relevance for infection, autoimmunity, and tumor immunology. Using a newly developed, genome-wide retroviral CRISPR knockout (KO) library, combined with RNA-seq, ATAC-seq, and ChIP-seq, we have dissected
Sydney Lavoie et al.
Gastroenterology, 158(5), 1359-1372 (2020-01-10)
Intestinal microbes and their metabolites affect the development of colorectal cancer (CRC). Short-chain fatty acids are metabolites generated by intestinal microbes from dietary fiber. We investigated the mechanisms by which free fatty acid receptor 2 (FFAR2), a receptor for short-chain

Artigos

Directed Evolution

An overview of directed evolution and the methods for generating proteins with optimized or entirely new functions.

Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.

Entre em contato com a assistência técnica