10197734001

Glutamate Dehydrogenase (GlDH)

from beef liver

• Sinônimo(s): DNA marker
• Número da licença da enzima: 1.4.1.3 (BRENDA, IUBMB)
• Código UNSPSC: 12352204

Propriedades

• fonte biológica: bovine liver
• Nível de qualidade: 100
• Formulário: lyophilized
• atividade específica: 10 U/mg; ~10 units/mg protein (at 25 °C with 2-oxoglutarate as the substrate, and ADP as the activator.)
• embalagem: pkg of 3,000 U
• fabricante/nome comercial: Roche
• concentração: ≥10-20 % (w/w)
• técnica(s): activity assay: suitable
• cor: white
• pH ideal: 8
• solubilidade: water: 20 mg/mL, colorless
• adequação: suitable for UV spectrophotometry and general use
• nº de adesão UniProt: P00366
• aplicação(ões): life science and biopharma
• atividade externa: ADH <0.00500%; LDH <0.00500%; MDH <0.00500%
• Condições de expedição: wet ice
• temperatura de armazenamento: 2-8°C
• Informações sobre genes: bovine ... GLUD1(281785)

Descrição

Descrição geral

Approximately 10 U/mg lyophilizate (120 U/mg enzyme protein) at +25°C with 2-oxoglutarate as the substrate, and ADP as the activator.

GDH (Glutamate dehydrogenase) is a hexamer of 449 residues and is located in the mitochondria. It acts as a branch-point enzyme between amino acid oxidation and urea production. L-glutamate:NAD(P)⁺ oxidoreductase is involved in deamination.

Aplicação

Glutamate dehydrogenase has been used to measure residual ammonium by enzymatic analysis during fermentation by wine yeast. It used as a component of cofactor recycling systems for NAD(P) and NAD(P)H.

Qualidade

Contaminants: <0.005% ADH, LDH, and MDH, each

Sequência

The smallest active structure of GIDH (310,000 to 350,000 ) is a hexamer subunit of 55,400 D. This hexamer reversibly self-assembles forming a polymer with up to eight hexamers (2,200 kD).

Outras notas

For life science research only. Not for use in diagnostic procedures.

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): does not flash
• Ponto de fulgor (°C): does not flash