This product is soluble at 1 mM in water. The Certificate of Analysis recommends using sterile distilled water. Tissue culture grade water is suitable.
SCM075
Inibidor de ROCK (Y-27632)
The ROCK Inhibitor (Y-27632) is available in a 5 mg format & has been optimized & validated for cell culture & Neuroscience applications.
Sinônimo(s):
Y-27632
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About This Item
Código UNSPSC:
12352207
eCl@ss:
32160801
NACRES:
NA.75
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
Nível de qualidade
Formulário
liquid
peso molecular
320.26 g/mol
técnica(s)
cell culture | mammalian: suitable
Condições de expedição
dry ice
Descrição geral
O inibidor de ROCK (Y-27632) é um inibidor competitivo com ATP e seletivo de quinases proteicas associadas a Rho (ROCK), incluindo a p160Rock (Ki=140nM) e a Rock-II. Também age como potente inibidor da sensibilização a Ca2+ induzida por agonista da fosforilação de miosina e contração de músculo liso. Um tratamento de uma hora com 10uM de Y-27632 bloqueia a apoptose de células-tronco embrionárias humanas (CTEh) cultivadas dissociadas, aumentando a sobrevivência e a eficiência de clonagem das CTEh sem afetar sua pluripotência1. Recentemente, alguns relatos demonstraram que a aplicação de Y-27632 e Accutase (N.º do cat. SCR005), uma mistura formulada de enzimas digestivas, aumenta a taxa de sobrevivência pós-descongelamento de CTEh individuais usando métodos convencionais de congelamento lento e descongelamento rápido
Armazenamento e estabilidade
Antes da reconstituição: Manter a -20 ºC, ao abrigo da luz por 2 anos. Após a reconstituição: 1 ano a -20 ºC, ao abrigo da luz
Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 3
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
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