S7117
ApopTag Plastic Coverslip
APOPTAG Plastic Coverslips are designed to be custom fitted to the specific APOPTAG kit application.
Sinônimo(s):
Plastic coverslip
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12161503
eCl@ss:
32161000
NACRES:
NA.32
Produtos recomendados
Nível de qualidade
fabricante/nome comercial
ApopTag
Chemicon®
técnica(s)
activity assay: suitable (apoptosis)
Condições de expedição
ambient
Descrição geral
APOPTAG Plastic Coverslips are designed to be custom fitted to the specific APOPTAG kit application. Each coverslip has 2.5" x 1" dimensions.
Aplicação
Plastic coverslips can be used to assure that a constant volume of solution is applied per unit of specimen area. However, their handling time slows down the protocol. Each square centimeter of plastic coverslip will require the volume of reagent indicated in Table 5 (S7100, S7101) and Table 10 (S7110, S7111, S7160), so that the reagent volume applied per unit of tissue area can be held constant. The surface to be covered is always equal to the area of the plastic coverslip, not the area of the specimen.
Plastic coverslips may be trimmed to any desired size and shape. The kit′s yield of specimens will be reduced if larger than standard coverslips are used.
When using ApopTag Kits that contain the anti-digoxigenin antibodies (S7100, S7101, S7110, S7111), plastic coverslips can be used during the incubation steps with the following reagents: WORKING STRENGTH TdT, and the ANTI-DIGOXIGENIN ANTIBODY. When using the ApopTag Fluorescein Direct Kit (S7160), the plastic coverslips can be used during incubation steps with WORKING STRENGTH TdT.
A basic coverslip method is described as follows:
To make a pair of "standard area" (~5 cm2) specimen coverslips, cut a plastic coverslip (provided) into two equal halves, and fold up a 1 cm handling tab across the width, then crease sharply (See Figure 3 in insert).
Drain one slide for approximately 10 seconds, and then tap off drops on a paper towel on the benchtop. Blot back and sides of the slide with a folded wipe. Carefully blot the area around the tissue section or cells, or else vacuum up solution using a pipette attached to an aspirator vacuum.
Apply reagent solution to one end of the area to be covered, using a dropper bottle or pipette as required.
Grasp the plastic coverslip by the handling tab and touch its opposite end to the droplet of reagent on the slide. Slightly arching the coverslip, roll it slowly downward, causing the solution to spread by capillary action. If solution does not spread evenly, tilt the slide until the flow reaches all edges.
Apply plastic coverslips to microscope slides so as to minimize trapped air bubbles, which may cause variable enzyme reaction or detection.
Place the slide across the pipettes, face-up and level, inside the humidified chamber. The slide edges should not touch anything so as to prevent drainage of the reagent.
Plastic coverslips may be trimmed to any desired size and shape. The kit′s yield of specimens will be reduced if larger than standard coverslips are used.
When using ApopTag Kits that contain the anti-digoxigenin antibodies (S7100, S7101, S7110, S7111), plastic coverslips can be used during the incubation steps with the following reagents: WORKING STRENGTH TdT, and the ANTI-DIGOXIGENIN ANTIBODY. When using the ApopTag Fluorescein Direct Kit (S7160), the plastic coverslips can be used during incubation steps with WORKING STRENGTH TdT.
A basic coverslip method is described as follows:
To make a pair of "standard area" (~5 cm2) specimen coverslips, cut a plastic coverslip (provided) into two equal halves, and fold up a 1 cm handling tab across the width, then crease sharply (See Figure 3 in insert).
Drain one slide for approximately 10 seconds, and then tap off drops on a paper towel on the benchtop. Blot back and sides of the slide with a folded wipe. Carefully blot the area around the tissue section or cells, or else vacuum up solution using a pipette attached to an aspirator vacuum.
Apply reagent solution to one end of the area to be covered, using a dropper bottle or pipette as required.
Grasp the plastic coverslip by the handling tab and touch its opposite end to the droplet of reagent on the slide. Slightly arching the coverslip, roll it slowly downward, causing the solution to spread by capillary action. If solution does not spread evenly, tilt the slide until the flow reaches all edges.
Apply plastic coverslips to microscope slides so as to minimize trapped air bubbles, which may cause variable enzyme reaction or detection.
Place the slide across the pipettes, face-up and level, inside the humidified chamber. The slide edges should not touch anything so as to prevent drainage of the reagent.
Armazenamento e estabilidade
Store at room temperature.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica