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Merck
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Documentos Principais

PC213L

Sigma-Aldrich

Anti-GABA Rabbit pAb

lyophilized, Calbiochem®

Sinônimo(s):

Anti-Gamma Amino-Butyric Acid

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About This Item

Código UNSPSC:
12352203
NACRES:
NA.43

fonte biológica

rabbit

Nível de qualidade

forma do anticorpo

serum

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

Formulário

lyophilized

não contém

preservative

reatividade de espécies

rat

fabricante/nome comercial

Calbiochem®

condição de armazenamento

OK to freeze

Isotipo

IgG

Condições de expedição

ambient

temperatura de armazenamento

−20°C

modificação pós-traducional do alvo

unmodified

Informações sobre genes

Descrição geral

Anti-GABA, rabbit polyclonal, recognizes GABA in rat thalamus and cerebellum. It is validated for use in immunohistochemistry on frozen sections.
Rabbit polyclonal antibody supplied as lyophilized undiluted serum. Recognizes GABA.
Recognizes GABA in rat thalamus and cerebellum.

Imunogênio

GABA
Rat

Aplicação


Frozen Sections (1:12,000-1:15,000, ABC detection or 1:3000-1:5000, PAP detection; see comments)

Advertência

Toxicity: Standard Handling (A)

forma física

Undiluted serum.

Reconstituição

Reconstitute the lyophilized antibody with 100 µl sterile distilled H₂O. For storage, dilute with sterile PBS or Tris buffer at dilutions no higher than 1:10. Reconstitute the entire contents of the vial; during shipment and handling portions of the lyophilized pellet may have become dislodged and may not be in the bottom of the vial. Following reconstitution, aliquot and freeze (-20°C). Stock solutions are stable for up to 6 months at -20°C. Avoid freeze/thaw cycles of solutions.

Nota de análise

Positive Control
Rat thalamus or cerebellum

Outras notas

Antibody specificity was examined in the rat thalamus and cerebellum. Glutaraldehyde is a necessary component of fixation for use with this antibody. Higher concentrations of glutaraldehyde (1%-2%) may be used as needed. The specificity of the antibody for GABA was evaluated using a competitive inhibition ELISA. A GABA conjugate control completely eliminated labeling, while 1000-fold excess of the following conjugates: alanine, aspartate, beta alanine, glutamate, glycine, taurine, and tyrosine did not inhibit the binding to the GABA conjugate control. Antibody should be titrated for optimal results in individual systems.
Sudhof, T.C. 1995. Nature375, 645.
Hokfelt, T., et al. 1987. Experientia Suppl.56, 154.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

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Código de classe de armazenamento

10 - Combustible liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


Certificados de análise (COA)

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