MABT1548

Anti-CD97 Antibody, clone CLB‐CD97/1

• Sinônimo(s): Adhesion G protein-coupled receptor E5, Leukocyte antigen CD97
• Código UNSPSC: 12352203
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 200
• forma do anticorpo: purified antibody
• tipo de produto de anticorpo: primary antibodies
• clone: CLB?CD97/1, monoclonal
• peso molecular: calculated mol wt 92 kDa; observed mol wt ~N/A kDa
• purificado por: using protein G
• reatividade de espécies: human
• embalagem: antibody small pack of 100
• técnica(s): ELISA: suitable; flow cytometry: suitable; immunofluorescence: suitable; immunohistochemistry: suitable; immunoprecipitation (IP): suitable; inhibition assay: suitable
• Isotipo: IgG2a
• sequência de epítopo: N-terminal extracellular domain
• nº de adesão de ID de proteína: NP_001020331
• nº de adesão UniProt: P48960
• temperatura de armazenamento: -10 to -25°C
• Informações sobre genes: human ... ADGRE5(976)

Descrição

Especificidade

Clone CLB‐CD97/1 is a mouse monoclonal antibody that detects CD97 (Adhesion G protein-coupled receptor E5).

Imunogênio

NIH3T3 cells stably expressing human CD97.

Aplicação

Quality Control Testing

Evaluated by Flow Cytometry in THP-1 cells.

Flow Cytometry Analysis: 1.0 µg of this antibody detected CD97 in one million THP-1 cells.

Tested Applications

Flow Cytometry Analysis: A representative lot detected CD97 in Flow Cytometry applications (Hamann, J., et al. (1996). 184(3):1185-9; Kwakkenbos, M.J., et al. (2002). J Leukoc Biol. 71(5):854-62).

Inhibition Assay: A representative lot of this antibody inhibited erythrocyte adhesion to CD97-transfected COS cells.(Hamann, J., et al. (1996). 184(3):1185-9; Hamann, J., et al. (1998). Eur J Immunol.;28(5):1701-7).

Immunoprecipitation Analysis: A representative lot immunoprecipitated CD97 in Immunoprecipitation applications (Hamann, J., et al. (1996). 184(3):1185-9).

Immunohistochemistry Applications: A representative lot detected CD97 in Immunohistochemistry applications (Hamann, J., et al. (1999). Arthritis Rheum. 42(4):650-8; Steinert, M., et al. (2002). Am J Pathol. 161(5):1657-67).

Immunofluorescence Analysis: A representative lot detected CD97 in Immunofluorescence applications (Hamann, J., et al. (1998). Eur J Immunol. 28(5):1701-7; Hamann, J., et al. (1999). Arthritis Rheum. 42(4):650-8; Steinert, M., et al. (2002). Am J Pathol. 161(5):1657-67).

ELISA Analysis: A representative lot detected CD97 in ELISA applications (Hamann, J., et al. (1999). Arthritis Rheum. 42(4):650-8; Steinert, M., et al. (2002). Am J Pathol. 161(5):1657-67).

Note: Actual optimal working dilutions must be determined by end user as specimens, and experimental conditions may vary with the end user.

Descrição-alvo

Adhesion G protein-coupled receptor E5 (UniProt: P48960; also known as Leukocyte antigen CD97, CD97) is encoded by the ADGRE5 (also known as CD97) gene (Gene ID: 976) in human. CD97 is a multi-pass, homodimeric, membrane glycoprotein of the G-protein coupled receptor 2 family that is synthesized with a signal peptide (aa 1-20), which is subsequently cleaved off to produce the mature protein that contains four extracellular domains, seven transmembrane domains, and four cytoplasmic domains. The extracellular domain contains five EGF-like domains. The second and third EGF-like domains contain calcium binding sites, which are important in certain ligand interactions that are calcium-dependent. The fourth EGF-like domain mediates binding of chondroitin sulfate. The extracellular domain also contains one Arg-Gly-Asp (RGD) motif that serves as a recognition site for integrin binding. CD97 levels are quickly upregulated upon activation of most leukocytes. It is involved in cell adhesion and migration through interactions with cell surface proteins and components of the extracellular matrix. Three ligands for CD97 have been recognized: CD55 that serves as a negative regulator of complement cascade; chondroitin sulfate, and integrin 5β1. CD97 is broadly expressed and is found on most hematopoietic cells, including activated lymphocytes, monocytes, macrophages, dendritic cells, and granulocytes. Its expression is also observed on smooth muscle cells. Higher expression of CD97 is reported in thyroid, colorectal, esophageal, and pancreatic carcinomas. Its expression levels correlate with dedifferentiation, migration, and invasion in tumor cells. Three isoforms of CD97 have been described that are produced by alternative splicing. (Ref.: Safaee, M., et al. (2013). Int. J. Oncology. 43(5); 1343-1350; Steinert, M., et al. (2002). Am. J. Pathol. 161(5); 1657-1667; Kwakkenbos, MJ., et al. (2002). J. leukocyte Biol. 71(5); 854-862; Hamann, J., et al. (1996). J. Exp. Med. 184(3); 1185-1189).

forma física

Purified mouse monoclonal antibody IgG2a in PBS without preservatives.

Reconstituição

1.0 mg/mL. Please refer to guidance on suggested starting dilutions and/or titers per application and sample type.

Armazenamento e estabilidade

Store at -10°C to -25°C. Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable