MABN385

Anti-NHERF-2 Antibody, clone 32B6

clone 32B6, from mouse

• Sinônimo(s): Na(+)/H(+) exchange regulatory cofactor NHE-RF2, NHERF-2, NHE3 kinase A regulatory protein E3KARP, SRY-interacting protein 1, SIP-1, Sodium-hydrogen exchanger regulatory factor 2, Solute carrier family 9 isoform A3 regulatory factor 2, Tyrosine kinase ac
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 32B6, monoclonal
• reatividade de espécies: rat, human
• técnica(s): immunocytochemistry: suitable; immunoprecipitation (IP): suitable; western blot: suitable
• Isotipo: IgG1
• nº de adesão NCBI: NP_001123484
• nº de adesão UniProt: Q15599
• Condições de expedição: wet ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... SLC9A3R2(9351)

Descrição

Descrição geral

Na(+)/H(+) exchange regulatory cofactor NHE-RF2 (NHERF-2) is also known as NHE3 kinase A regulatory protein E3KARP, SRY-interacting protein 1 (SIP-1), Sodium-hydrogen exchanger regulatory factor 2, Solute carrier family 9 isoform A3 regulatory factor 2 (SLC9A3R2), and Tyrosine kinase activator protein 1 (TKA-1). NHERF-2 is a Scaffold protein that links ezrin/moesin/radixin family members to the actin cytoskeleton and regulates their surface expression. NHERF-2 is critical for the cAMP-mediated phosphorylation and inhibition of SLC9A3. NHERF-2 is also thought to act as scaffold protein in the nucleus.

Imunogênio

recombinant protein corresponding to human NHERF-2.

Aplicação

Anti-NHERF-2 Antibody, clone 32B6 is a highly specific mouse monoclonal antibody, that targets NHERF2 & has been tested in western blotting, ICC & IP.

Immunocytochemistry Analysis: A representative lot was used by an independent laboratory in A431 and NHERF-2 transfected HT1080 cells. (Theisen, C. S., et al. (2007). Mole. Biol. Cell. 18:1220-1232).
Immunoprecipitation Analysis: A representative lot was used by an independent laboratory in Batt cell lysate. (Theisen, C. S., et al. (2007). Mole. Biol. Cell. 18:1220-1232).

Research Category
Neuroscience

Research Sub Category
Developmental Signaling

Qualidade

Evaluated by Western Blotting in HaCaT keratinocytes cell lysate.

Western Blotting Analysis: 1.0 µg/mL of this antibody detected NHERF-2 in 10 µg of HaCaT keratinocytes cell lysate.

Descrição-alvo

~ 37 kDa observed. An uncharacterized band may appear at ~41 kDa in some lysates.

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1 in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
HaCaT keratinocytes cell lysate

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 1
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable