MABF854

Anti-S100A9 Antibody, clone 6B4

clone 6B4, from mouse

• Sinônimo(s): Protein S100-A9, Calgranulin-B, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, MRP-14, p14, S100 calcium-binding protein A9, S100A9
• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: mouse
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: 6B4, monoclonal
• reatividade de espécies: human
• não deve reagir com: mouse
• técnica(s): ELISA: suitable; western blot: suitable
• Isotipo: IgG1κ
• nº de adesão NCBI: NP_002956
• nº de adesão UniProt: P06702
• Condições de expedição: dry ice
• modificação pós-traducional do alvo: unmodified
• Informações sobre genes: human ... S100A9(6280)

Descrição

Descrição geral

Protein S100-A9 (UniProt P06702; also known as Calgranulin-B, Calprotectin L1H subunit, Leukocyte L1 complex heavy chain, Migration inhibitory factor-related protein 14, MRP-14, p14, S100 calcium-binding protein A9, S100A9) is encoded by the S100A9 (also known as 60B8AG, CAGB, CFAG, CGLB, L1AG, LIAG, MAC387, MRP14) gene (Gene ID 6280) in human. S100A9 is one of the 21 members of the S100 family of proteins. S100A8 (calgranulin-A), S100A9 (calgranulin-B) and the heterdimer S100A8/S100A9 (calprotectin) exert their immunological and inflammatory action via binding to TLR-4, RAGE and some other putative receptors. S100A8, calprotectin, and S100A9 are chemoattractants for neutrophils, and S100A9 is known to induce the expression and activation of beta2-integrins. S100A9 is also shown to stimulate neutrophil degranulation and phagocytosis, while both S100A8 and S100A9 can induce the secretion of inflammatory cytokines from human monocytes. In addition, S100A9 is shown to potentiate both fMLP- and GM-CSF-stimulated IL-8 production from neutrophils, although S100A9 on its own cannot induce neutrophil cytokine production in the absence of fMLP and GM-CSF.

Especificidade

Clone 6B4 selectively detects human S100A9 with no cross-reactivity toward hS100A8, hS100A12, mS100A8, or mS100A9.

Imunogênio

Recombinant protein corresponding to human S100A9.

Aplicação

Research Category
Inflammation & Immunology

Research Sub Category
Immunoglobulins & Immunology

This mouse monoclonal Anti-S100A9 Antibody, clone 6B4, Cat. No. MABF854 is validated for use in Western Blotting and ELISA, for the detection of S100A9.

Western Blotting Analysis: A representative lot detected recombinant hS100A9 (rhS100A9), as well as endogenous hS100A9 in neutrophil extract and purified calprotectin (S100A8/S100A9 dimer), but not rhS100A8 & rhS100A12 or recombinant murine S100A8 & S100A9 (Dr Philippe Tessier, Laval University, Quebec, CA).
ELISA Analysis: A representative lot detected recombinant human S100A9 (rhS100A9) by direct/non-sandwich ELISA, but not rhS100A8 & rhS100A12 or recombinant murine S100A8 & S100A9 (Dr Philippe Tessier, Laval University, Quebec, CA).
ELISA Analysis: A representative lot detected recombinant human S100A9 (rhS100A9) as well as endogenous S100A9 in purified human calprotectin (S100A8/S100A9 dimer) by sandwich ELISA, but not rhS100A8 or rhS100A12 (Dr Philippe Tessier, Laval University, Quebec, CA).

Qualidade

Evaluated by Western Blotting in human spleen tissue lysate.

Western Blotting Analysis: 0.1 µg/mL of this antibody detected S100A9 in 10 µg of human spleen tissue lysate.

Descrição-alvo

~13 kDa observed

forma física

Format: Purified

Protein G Purified

Purified mouse monoclonal IgG1κ antibody in buffer containing PBS without preservatives.

Armazenamento e estabilidade

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.

Outras notas

Concentration: Please refer to lot specific datasheet.

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 12 - Non Combustible Liquids
• Classe de risco de água (WGK): WGK 2
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable