MABE343-AF488
Anti-Puromycin, clone 12D10, Alexa Fluor™ 488 Conjugate Antibody
clone 12D10, 0.5 mg/mL, from mouse
Sinônimo(s):
Alexa Fluor 488 antibody
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
conjugado
ALEXA FLUOR™ 488
forma do anticorpo
purified antibody
tipo de produto de anticorpo
primary antibodies
clone
12D10, monoclonal
reatividade de espécies
human
concentração
0.5 mg/mL
técnica(s)
immunocytochemistry: suitable
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Descrição geral
Puromycin is an aminonucleoside antibiotic, derived from the Streptomyces alboniger bacterium, that functions as a protein synthesis inhibitor that blocks translation through premature chain termination in the ribosome. Monoclonal antibodies to puromycin may be used with standard immunochemical methods to directly monitor translation, a method known as surface sensing of translation (SUnSET). Part of the molecule resembles the 3′ end of the aminoacylated tRNA, making it useful for protein translation analysis. Puromycin induces DNA fragmentation in thymocytes and in human HL-60 leukemia cells.
This product is a conjugated version of Cat. No. MABE343.
This product is a conjugated version of Cat. No. MABE343.
Especificidade
Demonstrated to react with Human test sample, preincubated with Puromycin. Predicted to react with all species when test sample is incubated with Puromycin.
This antibody detects Puromycin-incorporated neosynthesized proteins.
Imunogênio
Puromycin-incorporated neosynthesized proteins at multiple molecular weights
Aplicação
Research Category
Epigenetics & Nuclear Function
Epigenetics & Nuclear Function
Research Sub Category
Cell Cycle, DNA Replication & Repair
Cell Cycle, DNA Replication & Repair
This Anti-Puromycin, clone 12D10 is validated for use in Immunocytochemistry for the detection of Puromycin.
Qualidade
Evaluated by Immunocytochemistry in untreated and Puromycin treated HeLa cells.
Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.
Alexa Fluor™ is a registered trademark of Life Technologies.
Immunocytochemistry Analysis: A 1:2,500 dilution of this antibody detected Puromycin-incorporated neosynthesized proteins in HeLa cells treated with Puromycin only.
Alexa Fluor™ is a registered trademark of Life Technologies.
Descrição-alvo
Refer to Cat. No. MABE343 for observed molecular weight information.
forma física
Protein G Purified
Purified rat monoclonal conjugate in buffer containing PBS and 15 mg/ml BSA with 0.1% Sodium Azide.
Armazenamento e estabilidade
Stable for 1 year at 2-8°C from date of receipt.
Informações legais
ALEXA FLUOR is a trademark of Life Technologies
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Qi Wu et al.
Oxidative medicine and cellular longevity, 2019, 7064319-7064319 (2019-06-19)
Ribosome biogenesis is a crucial biological process related to cell proliferation, redox balance, and muscle contractility. Aortic smooth muscle cells (ASMCs) show inhibition of proliferation and apoptosis, along with high levels of oxidative stress in aortic dissection (AD). Theoretically, ribosome
Benjamin J Jenkins et al.
Cell metabolism, 35(7), 1132-1146 (2023-05-26)
Augmented T cell function leading to host damage in autoimmunity is supported by metabolic dysregulation, making targeting immunometabolism an attractive therapeutic avenue. Canagliflozin, a type 2 diabetes drug, is a sodium glucose co-transporter 2 (SGLT2) inhibitor with known off-target effects on
Sara Hosseini-Farahabadi et al.
PLoS biology, 19(5), e3001221-e3001221 (2021-05-04)
Premature termination codons (PTC) cause over 10% of genetic disease cases. Some aminoglycosides that bind to the ribosome decoding center can induce PTC readthrough and restore low levels of full-length functional proteins. However, concomitant inhibition of protein synthesis limits the
Danielle Minichino et al.
Journal of leukocyte biology, 112(5), 1089-1104 (2022-06-02)
Langerhans cell histiocytosis (LCH) is an inflammatory disease characterized by abnormal dendritic cells (DCs) with hyperactive ERK signaling, called "LCH cells." Since DCs rely on ERK signaling to produce inflammatory molecules in response to pathogenic cues, we hypothesized that hyperactive
Fangyuan Jia et al.
Oxidative medicine and cellular longevity, 2021, 5986260-5986260 (2021-01-30)
The rate of ribosome biogenesis plays a vital role in cell cycle progression and proliferation and is strongly connected with coronary restenosis and atherosclerosis. Blocking of proliferation 1 (BOP1) has been found as an evolutionarily conserved gene and a pivotal
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