The kit has been verified for use with CSF and cell/tissue culture supernatants. Protein normalization is not required for these sample types. If using the kit with cell or tissue lysates, the sample will need to be normalized to total protein, using either a BCA or Coomassie-based assay. The product has not been validated for use with cell/tissue lysates.
HNS1MAG-95K
MILLIPLEX® Human Neuroscience Magnetic Bead Panel 1 - Neuroscience Multiplex Assay
allows quantitative multiplex detection of multiple analytes simultaneously
About This Item
Produtos recomendados
Nível de qualidade
reatividade de espécies
human
fabricante/nome comercial
Milliplex®
assay range
accuracy: 107%
(DJ1)
accuracy: 108%
(a-Synuclein)
accuracy: 123%
(TG2)
standard curve range: 12-50,000 pg/mL
(GFAP)
standard curve range: 15-60,000 pg/mL
(NSE)
standard curve range: 20-80,000 pg/mL
(UCHL1)
standard curve range: 244-1,000,000 pg/mL
(alpha-synuclein)
standard curve range: 61-250,000 pg/mL
(DJ1)
standard curve range: 61-250,000 pg/mL
(TG2)
técnica(s)
multiplexing: suitable
método de detecção
fluorometric (Luminex xMAP)
Condições de expedição
ambient
Categorias relacionadas
Descrição geral
MILLIPLEX® Human Neuroscience Bead Panel 1 is a 6-plex kit to be used for the simultaneous quantification of any or all of the following analytes in cerebrospinal fluid (CSF) samples: α-Synuclein, DJ1/PARK7, Glial fibrillary acidic protein (GFAP), Neuron specific enolase (NSE), Transglutaminase 2 (TGM2), and Ubiquitin carboxyl-terminal esterase L1 (UCHL1/PARK5).
Panel Type: Neuroscience
Especificidade
Cross-reactivity between the antibodies and any of the other analytes in this panel is non-detectable or negligible.
Aplicação
- Analytes: GFAP, NSE, α-Synuclein, PARK5, Transglutaminase 2 (TGM2)
- Recommended Sample type: CSF
- Recommended Sample dilution: Neat
- Assay Run Time: Overnight or one day
- Research Category: Neuroscience
Características e benefícios
Embalagem
Armazenamento e estabilidade
Outras notas
Informações legais
Exoneração de responsabilidade
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How can I normalize protein for this test? Just using same volume and total concentration protein are not reliable.
1 answer-
Helpful?
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Can serum or plasma samples be utilized in the Human Neuroscience Panel 1 kit, considering that the protocol exclusively mentions CSF?
1 answer-
The kit has not been validated with serum/plasma samples. Eight normal plasma samples were tested at a 1:4 dilution. It was found that GFAP and UCHL1 will require more sensitivity.
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