GF35L
Anti-BDNF Mouse mAb (35928.11)
lyophilized, clone 35928.11, Calbiochem®
Sinônimo(s):
Anti-Brain Derived Neurotrophic Factor
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About This Item
Código UNSPSC:
12352203
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
primary antibodies
clone
35928.11, monoclonal
forma
lyophilized
não contém
preservative
reatividade de espécies
human
fabricante/nome comercial
Calbiochem®
condição de armazenamento
OK to freeze
avoid repeated freeze/thaw cycles
Isotipo
IgG1
Condições de expedição
ambient
temperatura de armazenamento
−20°C
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... BDNF(627)
Descrição geral
Anti-BDNF, mouse monoclonal, clone 35928.11, recognizes the ~20 kDa BDNF protein in brain extracts. It is validated for ELISA, Western blotting, immunohistochemistry, and neutralization studies.
Immunoaffinty purified mouse monoclonal antibody. Recognizes the ~20 kDa BDNF protein.
Recognizes the ~20 kDa BDNF protein in brain extracts. Clone 35928.11 was selected based on its ability to neutralize the biological activity of human recombinant BDNF.
Imunogênio
recombinant protein consisting of amino acids 129-247 of human BDNF
Aplicação
ELISA (0.5-1 µg/ml)
Immunoblotting (1-2 µg/ml)
Immunohistochemistry (5-15 µg/ml)
Neutralization Studies (see comments)
Immunoblotting (1-2 µg/ml)
Immunohistochemistry (5-15 µg/ml)
Neutralization Studies (see comments)
Advertência
Toxicity: Standard Handling (A)
forma física
Lyophilized from PBS, 5% trehalose.
Reconstituição
We recommend resuspending the lyophilized antibody with sterile PBS, pH 7.4, or sterile 20 mM Tris-saline (20 mM Tris containing 0.15 M NaCl), pH 7.4, to yield a final concentration of 100 µg/ml. Following reconstitution, aliquot and freeze (-20°C).
Outras notas
Riccio, A., et al. 1997. Science277 1097.
Sendtner, M., et al. 1996. Neurochem. Res.21, 831.
Barbacid, M. 1995. Ann. N.Y. Acad. Sci.766, 442.
Gotz, R., et al. 1994. Nature372, 266.
Snider, W.D. 1994. Cell77, 627.
Snider, W.D., et al. 1992. J. Neurobiol.23, 1231.
Sendtner, M., et al. 1996. Neurochem. Res.21, 831.
Barbacid, M. 1995. Ann. N.Y. Acad. Sci.766, 442.
Gotz, R., et al. 1994. Nature372, 266.
Snider, W.D. 1994. Cell77, 627.
Snider, W.D., et al. 1992. J. Neurobiol.23, 1231.
The full-length sequence for BDNF can be found under accession number NP_001700. This antibody has been selected for its ability to neutralize the biological activity of human recombinant BDNF. The exact concentration of antibody required to neutralize human recombinant BDNF activity is dependent on the cytokine concentration, cell type, growth conditions, and the type of activity studied. Suggested neutralization concentration required to yield one-half maximal inhibition of BDNF activity is approximately 5-15 µg/ml in the presence of 2.5 ng/ml of human recombinant BDNF, in a neuron survival assay using embryonic chick dorsal root ganglia neurons. The detection limit for human recombinant BDNF is ~300 ng/lane under non-reducing and reducing conditions. The detection for human recombinant BDNF is ~12.5 ng/ml. Based on immunoblotting and ELISA, this antibody exhibits less than 2% cross-reactivity with human recombinant β-NGF, human recombinant NT-3, and human recombinant NT-4. Antibody should be titrated for optimal results in individual systems.
Informações legais
CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
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Código de classe de armazenamento
11 - Combustible Solids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
A Markham et al.
The European journal of neuroscience, 20(5), 1189-1196 (2004-09-03)
Brain-derived neurotrophic factor (BDNF) governs both the selective survival of neurons during development and the experience-based regulation of synaptic strength throughout life. BDNF produced a concentration-dependent increase in the respiratory control index (RCI, a measure of the efficiency of respiratory
Natalia A Stefanova et al.
Aging, 8(11), 2713-2733 (2016-10-18)
Mitochondrial aberrations are observed in human Alzheimer's disease (AD) and in medical conditions that increase the risk of this disorder, suggesting that mitochondrial dysfunction may contribute to pathophysiology of AD. Here, using OXYS rats that simulate key characteristics of sporadic
Hugo Talbot et al.
Scientific reports, 10(1), 12572-12572 (2020-07-30)
Evading apoptosis and sustained survival signaling pathways are two central hallmarks of B-cell chronic lymphocytic leukemia (B-CLL) cells. In this regard, nurse-like cells (NLC), the monocyte-derived type 2 macrophages, deliver stimulatory signals via B-cell activating factor (BAFF), a proliferation-inducing ligand
Tinmarla F Oo et al.
Molecular and cellular neurosciences, 41(4), 440-447 (2009-05-05)
Brain-derived neurotrophic factor (BDNF) was the first purified molecule identified to directly support the development of mesencephalic dopamine neurons. However, its physiologic role has remained unknown. Based on patterns of expression, it is unlikely to serve as a target-derived neurotrophic
Milena Penkowa et al.
Journal of neuroscience research, 83(6), 974-984 (2006-02-24)
Brain injury and neuroinflammation are pathophysiologic contributors to acute and chronic neurologic disorders, which are progressive diseases not fully understood. Mammalian metallothioneins I and II (MT-I&II) have significant neuroprotective functions, but the precise mechanisms underlying these effects are still unknown.
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