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ABN91

Sigma-Aldrich

Anti-NeuN Antibody

from chicken, purified by affinity chromatography

Sinônimo(s):

RNA binding protein fox-1 homolog 3, Fox-1 homolog C

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41

fonte biológica

chicken

Nível de qualidade

100
200

forma do anticorpo

affinity isolated antibody

tipo de produto de anticorpo

primary antibodies

clone

polyclonal

purificado por

affinity chromatography

reatividade de espécies

mouse, rat

técnica(s)

immunocytochemistry: suitable
immunohistochemistry: suitable (paraffin)
western blot: suitable

Isotipo

IgY

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

Descrição geral

NeuN (RNA binding protein fox-1 homolog 3; Fox-1 homolog C) is a RNA-binding protein found exclusively in the nuclei of neuronal cells. It is a member of the evolutionarily conserved Fox-1 family and is mainly involved in splicing of RNA.

Especificidade

Other homologies: Human (94% sequence homology).
This antibody recognizes the N-terminus of NeuN.

Imunogênio

Epitope: N-terminus
GST-tagged recombinant protein corresponding to the N-terminus of mouse NeuN.

Aplicação

Immunocytochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in rat E18 cortex cells.

Immunohistochemistry Analysis: A 1:500 dilution from a representative lot detected NeuN in mouse brain, mouse hippocampus, and mouse frontal cortex tissues.
Research Category
Neuroscience
Research Sub Category
Developmental Neuroscience
This Anti-NeuN Antibody is validated for use in IHC(P), Western Blotting, ICC for the detection of NeuN.

Qualidade

Evaluated by Western Blot in mouse brain E16 tissue lysate.

Western Blot Analysis: 0.5 µg/mL of this antibody detected NeuN on 10 µg of mouse brain E16 tissue lysate.

Descrição-alvo

~45 kDa observed. Uncharacterized bands appear at ~40 and ~50 kDa in some lysates.

forma física

Affinity purified
Purified chicken polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide.

Armazenamento e estabilidade

Stable for 1 year at 2-8°C from date of receipt.

Nota de análise

Control
Mouse brain E16 tissue lysate

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable


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