Good at showing doublet bands for the AQP4 isoforms by Western blot, 1:1000 goes a long way. Fair bit of non-specific binding at higher molecular weights which originally messed with the chemiluminescence signal when I was optimising, but this became moot when I stated cutting the membrane to load beta-actin and AQP4 antibodies seperately (at the same time). Would not reccomend trying 1:2000 primary or using NFM to block/dilute, not sure how good it is on PVDF membranes but worked well on my nitorcellulose.
AB3594
Anti-Aquaporin-4 Antibody
Anti-Aquaporin 4 Antibody, CT
CHEMICON®, rabbit polyclonal
Sinônimo(s):
Mercurial-Insensitive Water Channel
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Preço e disponibilidade não estão disponíveis no momento.
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Nome do produto
Anti-Aquaporin 4 Antibody, CT, Chemicon®, from rabbit
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
affinity isolated antibody
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
reatividade de espécies
mouse, human, rat
fabricante/nome comercial
Chemicon®
técnica(s)
immunohistochemistry: suitable
western blot: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... AQP4(361)
Descrição geral
Water is a critical component of all living cells. Interestingly, tissue membranes show a great degree of water permeability. Mammalian red cells, renal proximal tubules, and descending thin limb of Henle are extraordinarily permeable to water. Water crosses hydrophobic plasma membranes either by simple diffusion or through a facilitative transport mechanism mediated by special proteins "aquaporins". Aquaporins, are integral membrane proteins from a larger family of major intrinsic proteins (MIP) that form pores in the membrane of biological cells. Over the last decade, genes for several members of aquaporin family have been cloned, expressed, and their distribution studied in many tissues. The water-specific channel Aquaporin 4 is located in various tissues including the gastrointestinal tract, kidney and brain. It is an osmoreceptor which regulates body water balance and mediates water flow within the central nervous system. In CNS tissue, AQP4 localizes to astrocyte endfeet making it a useful structural marker.
Especificidade
Reactivity with other species has not yet been tested. It is expected that the antibody may also react with bovine (71/75) and rabbit (64/75) due to sequence homology.
Recognizes Aquaporin 4 (AQP4, WCH4, Mercurial-Insensitive Water Channel).
Imunogênio
Epitope: C-terminus
GST fusion protein with residues 249-323 of rat Aquaporin 4 (SwissProt accession number P47863).
Aplicação
Detect Aquaporin 4 using this Anti-Aquaporin 4 Antibody, C-terminus validated for use in IH & WB.
Immunohistochemistry:
A previous lot of this antibody was used on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Western blotting:
1:500-1:1,000 using ECL on rat brain membranes.
Optimal working dilutions must be determined by the end user
A previous lot of this antibody was used on rat brain sections.
Dilutions should be made using a carrier protein such as BSA (1-3%).
Western blotting:
1:500-1:1,000 using ECL on rat brain membranes.
Optimal working dilutions must be determined by the end user
Research Category
Neuroscience
Neuroscience
Research Sub Category
Ion & Transport Channels
Ion & Transport Channels
Qualidade
Routinely evaluated by Western Blot on Mouse Brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected AQUAPORIN 4 on 10 μg of Mouse Brain lysates.
Western Blot Analysis: 1:500 dilution of this lot detected AQUAPORIN 4 on 10 μg of Mouse Brain lysates.
Descrição-alvo
38 kDa
forma física
Format: Purified
ImmunoAffinity Purified: Amber vial of Rb X Aquaporin 4, C-Terminus (part # AB3594-50ULa)
Purified rabbit polyclonal, lyophilized from buffer containing PBS, pH 7.4, containing 1% BSA, and 0.05% sodium azide. Reconstitute with 200 µL of sterile distilled water. Centrifuge antibody preparation before use (10,000 x g for 5 min).
Armazenamento e estabilidade
Stable for up to 6 months at -20ºC from date of receipt. After reconstitution stable at -20ºC in undiluted aliquots for up to 6 months. Avoid repeated freeze/thaw cycles.
Nota de análise
Control
Clear vial of RbX Aquaporin 4, CT Control Antigen(AB3594-50ULb) included free of charge with the antibody is 120 µg of control antigen (lyophilized powder). Reconstitute with 100 µL of PBS. For positive control, in Western blot use 20 ng of protein per Minigel lane. For negative control, preincubate 3 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Clear vial of RbX Aquaporin 4, CT Control Antigen(AB3594-50ULb) included free of charge with the antibody is 120 µg of control antigen (lyophilized powder). Reconstitute with 100 µL of PBS. For positive control, in Western blot use 20 ng of protein per Minigel lane. For negative control, preincubate 3 µg of purified peptide with 1 µg of antibody for one hour at room temperature. Optimal concentrations must be determined by the end user.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
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Nature communications, 11(1), 4411-4411 (2020-09-04)
The glymphatic system is a network of perivascular spaces that promotes movement of cerebrospinal fluid (CSF) into the brain and clearance of metabolic waste. This fluid transport system is supported by the water channel aquaporin-4 (AQP4) localized to vascular endfeet
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Active Filters
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Good Job (one optimised) - but be careful of non-specific binding
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