AB3579

Anti-BimEL (pS69) human / (pS65) rat Antibody

Chemicon^®, from rabbit

• Código UNSPSC: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

Propriedades

• fonte biológica: rabbit
• Nível de qualidade: 100
• forma do anticorpo: purified immunoglobulin
• tipo de produto de anticorpo: primary antibodies
• clone: polyclonal
• purificado por: affinity chromatography
• reatividade de espécies: rat, human
• reatividade da espécie (prevista por homologia): mouse (100% homologous)
• fabricante/nome comercial: Chemicon^®
• técnica(s): western blot: suitable
• nº de adesão NCBI: NM_006538.3; NM_138621.3; NM_138622.2; NM_138623.2; NM_138624.2; NM_138626.2; NM_138627.2; NM_207002.2; NM_207003.1
• nº de adesão UniProt: O43521
• Condições de expedição: dry ice
• modificação pós-traducional do alvo: phosphorylation (pSer69)
• Informações sobre genes: human ... BCL2L11(10018)

Descrição

Descrição geral

Bim (bcl-2-interacting mediator of cell death) is a proapoptotic member of the Bcl-2 family that shares only the BH3 domain with this family. There are three isoforms of Bim: Bim_EL, Bim_L, and Bim_S. Bim is involved in regulating the intrinsic mitochondrial apoptotic pathway by inducing cytochrome c release, which in turn, activates caspase-9 and then caspase-3. Bim also plays a critical role in central and peripheral deletion of T lymphocytes and in controlling B cell homeostasis and activation. Bim_EL, the long isoform of Bim, is a ~28 kDa protein that is predominantly expressed in T and B cells and is activated by ERK1/2 pathway. The activation of Bim_EL by ERK1/2 promotes its phosphorylation on serine 65 (serine 69 in human), targeting it for degradation via the proteasome.

Especificidade

Rat Bim_EL when phosphorylated at serine 65. Human Bim_EL when phosphorylated at serine 69. Mouse Bim_EL (100% conserved) has not been tested, but is expected to react.

Imunogênio

The antiserum was produced against a chemically synthesized phosphopeptide derived from the region of rat Bim_EL that contains serine 65 (serine 69 in the human sequence). The sequence is conserved in human and mouse.

Aplicação

Anti-BimEL (pS69) human / (pS65) rat Antibody is an antibody against BimEL (pS69) human / (pS65) rat for use in WB.

For Western blotting applications, we recommend using the antibody at a 1:1000 dilution.

Research Category
Apoptosis & Cancer

Research Sub Category
BCL2 & Inhibition

Qualidade

Purified from rabbit serum by sequential epitope-specific chromatography. The antibody has been negatively preadsorbed using a non-phosphopeptide corresponding to the site of phosphorylation to remove antibody that is reactive with non-phosphorylated Bim. The final product is generated by affinity chromatography using a Bim_EL-derived peptide that is phosphorylated at serine 65 (serine 69 in the human sequence).

forma física

Dulbecco′s phosphate buffered saline (without Mg²⁺ and Ca²⁺), pH 7.3 (+/- 0.1), 50% glycerol, with 1.0 mg/mL BSA (IgG, protease free) as a carrier. 0.05% sodium azide

Armazenamento e estabilidade

Store at -20°C. We recommend a brief centrifugation before opening to settle vial contents. Then, apportion into working aliquots and store at -20°C. For shipment or short-term storage (up to one week), 2-8°C is sufficient.

Nota de análise

Control
Hek293T co-expressing rat Bim_EL and active MEKK1 (MEKK1δ).

Informações legais

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Exoneração de responsabilidade

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Informações de segurança

• Código de classe de armazenamento: 10 - Combustible liquids
• Classe de risco de água (WGK): WGK 2