AB15348
Anti-FMRFamide Antibody
serum, Chemicon®
Sinônimo(s):
Anti-Anti-FMRFAL
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Preço e disponibilidade não estão disponíveis no momento.
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fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
serum
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
reatividade de espécies
rat
fabricante/nome comercial
Chemicon®
técnica(s)
immunohistochemistry: suitable
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
dry ice
modificação pós-traducional do alvo
unmodified
Imunogênio
FMRFamide
Aplicação
Anti-FMRFamide Antibody is an antibody against FMRFamide for use in IH.
Immunohistochemistry: 1:1,000 - 1:5,000
Application Notes for Immunohistochemistry:
- Suggested perfusion steps:
1) Ca2+-free Tyrode′s solution
2) Paraformaldehyde-picric acid fixative
3) 10% sucrose in PBS as a cryo-protectant
- Rapidly dissect tissue and store overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
- Incubate slide-mounted tissue sections with blocking buffer for 1 hour at room temperature.
- Remove blocking buffer and incubate the slides with the primary antibody (AB15348 diluted to desired working concentration in blocking buffer) for 18-24 hours at 2-8°C.
- Remove primary antibody and rinse 3 times in PBS.
- Incubate sections with fluorescently labeled secondary antibody for 60 minutes at room temperature.
Optimal working dilutions must be determined by the end user.
Application Notes for Immunohistochemistry:
- Suggested perfusion steps:
1) Ca2+-free Tyrode′s solution
2) Paraformaldehyde-picric acid fixative
3) 10% sucrose in PBS as a cryo-protectant
- Rapidly dissect tissue and store overnight in 0.1 M phosphate buffer (pH 7.4) containing 10% sucrose.
- Incubate slide-mounted tissue sections with blocking buffer for 1 hour at room temperature.
- Remove blocking buffer and incubate the slides with the primary antibody (AB15348 diluted to desired working concentration in blocking buffer) for 18-24 hours at 2-8°C.
- Remove primary antibody and rinse 3 times in PBS.
- Incubate sections with fluorescently labeled secondary antibody for 60 minutes at room temperature.
Optimal working dilutions must be determined by the end user.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Hormones & Receptors
Hormones & Receptors
forma física
Liquid with 0.05% sodium azide.
Serum
Armazenamento e estabilidade
Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 1
Certificados de análise (COA)
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