475911

Myeloperoxidase, Human Polymorphonuclear Leukocytes

A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins.

• Sinônimo(s): Myeloperoxidase, Human Polymorphonuclear Leukocytes, MPO
• Número CAS: 9003-99-0
• Número da licença da enzima: 1.11.1.7 (BRENDA, IUBMB)
• Número MDL: MFCD00071339
• Código UNSPSC: 12352202
• NACRES: NA.25

Propriedades

• fonte biológica: human
• Nível de qualidade: 200
• Ensaio: ≥95% (SDS-PAGE)
• Formulário: lyophilized
• atividade específica: 150-200 units/mg protein
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze
• solubilidade: water: soluble (volume is lot-specific and can be found on the product label)
• Condições de expedição: wet ice
• temperatura de armazenamento: −20°C
• cadeia de caracteres SMILES: [O+H2]O[O-]
• InChI: 1S/H2O3/c1-3-2/h1-2H
• chave InChI: JSPLKZUTYZBBKA-UHFFFAOYSA-N

Descrição

Descrição geral

Native, human myeloperoxidase from polymorphonuclear leukocytes. A lysosomal enzyme that catalyzes oxidations by hydrogen peroxide, including MPO-chloride-mediated killing of microbes and tumor cells, inactivation of chemotactic factors, and cross-linking of proteins. MPO catalyzes the formation of hypochlorous acid that kills bacteria either by binding the halide ion to intracellular constituents or by oxidation of bacterial lipids and proteins.

Advertência

Toxicity: Standard Handling (A)

Definição da unidade

One unit is defined as the amount of enzyme that will reduce 1.0 µmol H₂O₂ per min at 25°C, pH 6.0.

forma física

Lyophilized from 100-700 mM NaCl, 50 mM sodium acetate buffer, pH 6.0.

Nota de preparo

Further dilute in 100 mM NaCl, 50 mM sodium acetate, pH 6.0. Enzyme may precipitate out of solution in low ionic strength buffers or in distilled H₂O.

Reconstituição

Following reconstitution, aliquot and freeze (-20°C or -70°C). Stock solutions are stable for up to 1 year at -20°C or -70°C.

Outras notas

Nauseef, W.M., et al. 1998. J. Leukocyte Biol. 63, 264.
Panasenko, O.M., et al. 1994. Free Radic. Biol. Med. 16, 143.
Mulligan, M.S., et al. 1992. Br. J. Pharmacol.107, 1159.
Sharonov, B.P., and Churilova, I.V. 1992. Biochem. Biophys. Res. Commun.189, 1129.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Pictogramas: GHS08
• Palavra indicadora: Danger
• Frases de perigo: H334
• Declarações de precaução: P261 - P284 - P501
• Classificações de perigo: Resp. Sens. 1
• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 1