324740

Elastase Substrate V, Fluorogenic

≥95% (HPLC), powder, lyophilized solid, elastase substrate, Calbiochem^®

• Sinônimo(s): Elastase Substrate V, Fluorogenic, MeOSuc-AAPV-AMC
• Fórmula empírica (Notação de Hill): C₃₁H₄₁N₅O₉
• Número CAS: 72252-90-5
• Peso molecular: 627.69
• Número MDL: MFCD00077135
• Código UNSPSC: 12352200
• NACRES: NA.25

Propriedades

• product name: Elastase Substrate V, Fluorogenic, Elastase Substrate V, Fluorogenic, CAS 72252-90-5, is a sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM).
• Nível de qualidade: 200
• Ensaio: ≥95% (HPLC); ≥95% (TLC)
• forma: lyophilized solid; powder
• fabricante/nome comercial: Calbiochem^®
• condição de armazenamento: OK to freeze; desiccated; protect from light
• cor: white to off-white
• solubilidade: ethanol: 1 mg/mL; DMF: 5 mg/mL; DMSO: 5 mg/mL
• fluorescência: λ_ex 360-380 nm; λ_em 430-460 nm
• Condições de expedição: ambient
• temperatura de armazenamento: −20°C
• InChI: 1S/C31H41N5O9/c1-16(2)27(34-20-9-10-21-17(3)14-26(39)45-23(21)15-20)30(42)35-29(41)22-8-7-13-36(22)31(43)19(5)33-28(40)18(4)32-24(37)11-12-25(38)44-6/h9-10,14-16,18-19,22,27,34H,7-8,11-13H2,1-6H3,(H,32,37)(H,33,40)(H,35,41,42)/t18-,19-,22-,27-/m0/s1
• chave InChI: NHMQNKPYBPDCAY-NFHWZJRKSA-N

Descrição

Descrição geral

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte (K_m = 140 µM; K_cat/K_m = 120,000 s^-1 M^-1) and porcine pancreatic elastase. Not hydrolyzed by cathepsin G. Excitation max.: ~380 nm; emission max.: ~460 nm.

Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase (K_m = 140 µM; k_cat/K_m = 120,000 M^-1 s^-1). Not hydrolysed by cathepsin G.

Ações bioquímicas/fisiológicas

Cell permeable: no

Km = 140 µM; kcat/Km = 120,000 M⁻¹ s⁻¹as substrates for human leukocyte and porcine pancreatic elastase

Primary Target
Sensitive fluorogenic substrate for the quantitative determination of human leukocyte and porcine pancreatic elastase

Product does not compete with ATP.

Reversible: no

Advertência

Toxicity: Standard Handling (A)

Sequência

MeOSuc-Ala-Ala-Pro-Val-AMC

Reconstituição

Following reconstitution, aliquot and freeze at -20°C. Stock solutions are stable for up to 2 months at -20°C.

Outras notas

Barrett, A.J. 1981. Methods Enzymol. 80, 581.
Castillo, M.J., et al. 1979. Anal. Biochem.99, 53.
Nakajima, K., et al. 1979. J. Biol. Chem.254, 4027.

Informações legais

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Informações de segurança

• Código de classe de armazenamento: 11 - Combustible Solids
• Classe de risco de água (WGK): WGK 3
• Ponto de fulgor (°F): Not applicable
• Ponto de fulgor (°C): Not applicable