14-173-M
MAP Kinase 2/Erk2 Protein, active, mouse, 10 µg
Active, N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells, activated using MEK1 (Catalog #14-429). For use in Kinase Assays.
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About This Item
Código UNSPSC:
12352202
eCl@ss:
32160405
NACRES:
NA.41
Preço e disponibilidade não estão disponíveis no momento.
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
recombinante
expressed in E. coli
peso molecular
Mw 67.8 kDa
fabricante/nome comercial
Upstate®
técnica(s)
activity assay: suitable (kinase)
nº de adesão NCBI
nº de adesão UniProt
Descrição geral
N-terminal GST-tagged, recombinant MAP Kinase 2, expressed in E. coli cells; activated using MEK1 (Catalog #14-429).
Product Source: recombinant, mouse, full-length MAP Kinase 2
Ações bioquímicas/fisiológicas
Protein Target: MAPK2
Target Sub-Family: CMGC
Embalagem
Also available in 250 µg size --call for pricing and availability and reference catalog number 14-173M when ordering the 250 µg size.
Qualidade
Routinely evaluated by phosphorylation myelin basic protein
forma física
50mM Tris-HCl, pH 7.5, 150mM NaCl, 0.1mM EGTA, 0.03% Brij-35, 50% glycerol, 1mM benzamidine, 0.2mM PMSF, 0.1% 2-mercaptoethanol.
Glutathione agarose; Ni2+/NTA agarose
Armazenamento e estabilidade
Store in undiluted aliquots at -20°C for up to one year from the date of receipt. Avoid repeatede freeze-thaw cycles.
Outras notas
For Specific Activity data, refer to the Certificate of Analysis for individual lots of this enzyme.
Informações legais
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Palavra indicadora
Warning
Frases de perigo
Declarações de precaução
Classificações de perigo
Skin Sens. 1
Código de classe de armazenamento
10 - Combustible liquids
Classe de risco de água (WGK)
WGK 2
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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Purification and characterization of a maturation-activated myelin basic protein kinase from sea star oocytes.
Sanghera, J S, et al.
The Journal of Biological Chemistry, 265, 52-57 (1990)
X Yang et al.
The Journal of biological chemistry, 273(45), 29879-29887 (1998-10-29)
The human immunodeficiency virus type 1 (HIV-1) Vif protein plays a critical role in virus replication and infectivity. Here we show that Vif is phosphorylated and regulated by p44/42 mitogen-activated protein kinase (MAPK). Vif phosphorylation by MAPK was demonstrated in
Identification of insulin-stimulated protein kinase-1 as the rabbit equivalent of rskmo-2. Identification of two threonines phosphorylated during activation by mitogen-activated protein kinase.
Sutherland, C, et al.
European Journal of Biochemistry, 212, 581-588 (1993)
Tyrosyl phosphorylation and activation of the myelin basic protein kinase p44mpk during sea star oocyte maturation.
Sanghera, J S, et al.
Biochimica et Biophysica Acta, 1095, 153-160 (1991)
C Zhang et al.
The Journal of biological chemistry, 272(20), 13397-13402 (1997-05-16)
Aggregation of the high affinity IgE receptor (FcepsilonRI) in a mast cell line resulted in activation of the p42 and the stress-activated p38 mitogen-activated protein (MAP) kinases. Selective inhibition of these respective kinases with PD 098059 and SB 203580 indicated
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