MAB1998Z
Anti-Integrin α2β1 Antibody, clone BHA2.1, azide free
clone BHA2.1, Chemicon®, from mouse
Sinônimo(s):
VLA-2, MAB1998
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About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
mouse
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
BHA2.1, monoclonal
reatividade de espécies
human, pig
fabricante/nome comercial
Chemicon®
técnica(s)
flow cytometry: suitable
immunohistochemistry: suitable
immunoprecipitation (IP): suitable
Isotipo
IgG1κ
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
human ... ITGA2(3673)
Especificidade
Reacts with the collagen receptor VLA-2 (alpha2beta1) integrin complex.
Aplicação
Research Category
Cell Structure
Cell Structure
Research Sub Category
Integrins
Integrins
This Anti-Integrin α2β1 Antibody, clone BHA2.1, azide free is validated for use in FC, IH, IP, FUNC for the detection of Integrin α2β1.
flow cytometry: 10 μg/mL
Immunoprecipitation: Immunoprecipitation of VLA-2 integrins from lysate of 10E6 cell equivalent requires 2-5 μg of BHA2.1
immunohistochemistry in frozen tissue sections: 5 μg/mL
Does not detect alpha2 integrin subunit by Western blot presumably due to conformational properties of the epitope involved. The epitope for BHA2.1 is dependent on the presence of the (I) domain of alpha2 integrin subunit and does not bind VLA-2 variant which lacks the I-domain.
Function Blocking: BHA2.1 inhibits VLA-2 mediated cell adhesion to collagen and laminin; complete inhibition can generally be achieved at a final concentration of 10 μg/mL.
Final working dilutions must be determined by end user.
Immunoprecipitation: Immunoprecipitation of VLA-2 integrins from lysate of 10E6 cell equivalent requires 2-5 μg of BHA2.1
immunohistochemistry in frozen tissue sections: 5 μg/mL
Does not detect alpha2 integrin subunit by Western blot presumably due to conformational properties of the epitope involved. The epitope for BHA2.1 is dependent on the presence of the (I) domain of alpha2 integrin subunit and does not bind VLA-2 variant which lacks the I-domain.
Function Blocking: BHA2.1 inhibits VLA-2 mediated cell adhesion to collagen and laminin; complete inhibition can generally be achieved at a final concentration of 10 μg/mL.
Final working dilutions must be determined by end user.
forma física
Format: Purified
Protein A purified
Purified immunoglobulin from Protein A chromatography. Liquid in 0.02M PB pH 7.6, 0.25M NaCl containing no preservative.
Armazenamento e estabilidade
Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
Nota de análise
Control
Skin, CD4 & CD8 positive T cells
Skin, CD4 & CD8 positive T cells
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 2
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
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