06-574
Anti-TrkA Antibody
Upstate®, from rabbit
Sinônimo(s):
Neurotrophic tyrosine kinase receptor type, Oncogene TRK, TRK1-transforming tyrosine kinase protein, high affinity nerve growth factor receptor, neurotrophic tyrosine kinase, receptor, type 1, tyrosine kinase receptor A
Faça loginpara ver os preços organizacionais e de contrato
About This Item
Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Produtos recomendados
fonte biológica
rabbit
Nível de qualidade
forma do anticorpo
purified immunoglobulin
tipo de produto de anticorpo
primary antibodies
clone
polyclonal
reatividade de espécies
human, mouse, rat
fabricante/nome comercial
Upstate®
técnica(s)
immunocytochemistry: suitable
immunoprecipitation (IP): suitable
western blot: suitable
Isotipo
IgG
nº de adesão NCBI
nº de adesão UniProt
Condições de expedição
wet ice
modificação pós-traducional do alvo
unmodified
Informações sobre genes
rat ... Ntrk1(59109)
Descrição geral
TrkA, apparent molecular weight 140 kDa, is a high affinity nerve growth factor (NGF). The Trk proto-oncogene family contains four members, TrkA, TrkB, TrkC, and TrkE, which are variably expressed throughout the central and peripheral nervous systems. TrkA binds to nerve growth factor (NGF) and autophosphorylates on tyrosine residues (Tyr490, Tyr674, Tyr675, Tyr751 and Tyr785) to activate multiple downstream effector proteins. Phosphorylation at Tyr490 is required for Shc association and subsequent activation of the Ras-MAP kinase-signaling cascade, which leads to activation of Elk-1-dependent gene transcription and neurite growth. Phosphorylations at Tyr674 and Tyr675 lie within the catalytic domain of TrkA tyrosine kinase and reflect Trk kinase activity. Additionally, phosphorylation at Tyr751 is required for PI3-kinase association and activation of the Akt signaling cascade.
Especificidade
Reactivity with other species has not been confirmed.
Recognizes TrkA, Mr 140 kDa. Does not cross-react with TrkB or TrkC.
Imunogênio
Rabbits immunized with bacterially expressed purified protein corresponding to the entire extracellular domain of rat TrkA receptor.
Aplicação
Anti-TrkA Antibody detects level of TrkA & has been published & validated for use in IC, IP & WB.
Immunoprecipitation:
4 μg of a previous lot immunoprecipitated TrkA from 500 μg of PC-12 RIPA lysate.
Immunocytochemistry:
10 μg/mL of a previous lot of this antibody showed positive immunostaining for TrkA in PC-12 cells fixed with 4% paraformaldehyde.
4 μg of a previous lot immunoprecipitated TrkA from 500 μg of PC-12 RIPA lysate.
Immunocytochemistry:
10 μg/mL of a previous lot of this antibody showed positive immunostaining for TrkA in PC-12 cells fixed with 4% paraformaldehyde.
Research Category
Neuroscience
Neuroscience
Research Sub Category
Neurotransmitters & Receptors
Neurodegenerative Diseases
Neurotransmitters & Receptors
Neurodegenerative Diseases
Qualidade
Routinely evaluated by Western Blot on RIPA lysates from human A431 cells, mouse 3T3 or rat PC-12 cell lysates.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected TrkA in RIPA lysates from human A431 cells; a previous lot detected TrkA in mouse 3T3 and rat PC-12 cell lysates.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected TrkA in RIPA lysates from human A431 cells; a previous lot detected TrkA in mouse 3T3 and rat PC-12 cell lysates.
Descrição-alvo
140 kDa
Ligação
Replaces: CBL770
forma física
Format: Purified
Protein A purified
Purified rabbit IgG in buffer containing 0.02 M phosphate buffer, pH 7.6, 0.25 M NaCl, 0.1% sodium azide. Frozen at -20°C.
Armazenamento e estabilidade
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerolcontaining solutions to become frozen during storage.
Nota de análise
Control
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Positive Antigen Control: Catalog #12-301, non-stimulated A431 cell lysate. Add 2.5µL of 2-mercaptoethanol/100µL of lysate and boil for 5 minutes to reduce the preparation. Load 20µg of reduced lysate per lane for minigels.
Outras notas
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
Informações legais
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
Exoneração de responsabilidade
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
Not finding the right product?
Try our Ferramenta de seleção de produtos.
Código de classe de armazenamento
12 - Non Combustible Liquids
Classe de risco de água (WGK)
WGK 1
Ponto de fulgor (°F)
Not applicable
Ponto de fulgor (°C)
Not applicable
Certificados de análise (COA)
Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.
Já possui este produto?
Encontre a documentação dos produtos que você adquiriu recentemente na biblioteca de documentos.
Establishment of neurovascular congruency in the mouse whisker system by an independent patterning mechanism.
Oh, WJ; Gu, C
Neuron null
Nerve growth factor in the developing and adult lacrimal glands of rat with and without inherited retinitis pigmentosa.
Muzi S, Colafrancesco V, Sornelli F, Mantelli F, Lambiase A, Aloe L
Cornea null
An alternatively spliced form of the nerve growth factor receptor TrkA confers an enhanced response to neurotrophin 3
Clary, D. O. and Reichardt, L. F.
Proceedings of the National Academy of Sciences of the USA, 91, 11133-11137 (1994)
The Gata3 transcription factor is required for the survival of embryonic and adult sympathetic neurons.
Tsarovina, K; Reiff, T; Stubbusch, J; Kurek, D; Grosveld, FG; Parlato, R; Schutz, G; Rohrer, H
The Journal of Neuroscience null
Rui Ji et al.
PloS one, 9(12), e115140-e115140 (2014-12-17)
Tyro3, Axl and Mertk (TAM) receptor tyrosine kinases play multiple functional roles by either providing intrinsic trophic support for cell growth or regulating the expression of target genes that are important in the homeostatic regulation of immune responses. TAM receptors
Nossa equipe de cientistas tem experiência em todas as áreas de pesquisa, incluindo Life Sciences, ciência de materiais, síntese química, cromatografia, química analítica e muitas outras.
Entre em contato com a assistência técnica