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05-1572

Sigma-Aldrich

Anti-MDC1 Antibody, clone P2B11

clone P2B11, from mouse

Sinônimo(s):

Nuclear factor with BRCT domains 1, homologue to Drosophila photoreceptor protein calphotin, mediator of DNA damage checkpoint 1

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About This Item

Código UNSPSC:
12352203
eCl@ss:
32160702
NACRES:
NA.41
Preço e disponibilidade não estão disponíveis no momento.

fonte biológica

mouse

Nível de qualidade

100

forma do anticorpo

purified immunoglobulin

tipo de produto de anticorpo

primary antibodies

clone

P2B11, monoclonal

reatividade de espécies

mouse

reatividade da espécie (prevista por homologia)

chimpanzee (based on 100% sequence homology), bovine (based on 100% sequence homology), human (based on 100% sequence homology)

técnica(s)

immunocytochemistry: suitable
western blot: suitable

Isotipo

IgG1κ

nº de adesão NCBI

NP_001010833.2

nº de adesão UniProt

Q5PSV9

Condições de expedição

wet ice

modificação pós-traducional do alvo

unmodified

Informações sobre genes

human ... MDC1(9656)
mouse ... Mdc1(240087)

Descrição geral

MDC1 is a subunit of the cohesin complex and is an essential protein required for sister chromatid cohesion in mitosis and meiosis. Temperature-sensitive mutations of this protein have been associated with defects in sister chromatid cohesion, chromosome condensation, and chromosome segregation. Its expression is cell cycle regulated and peaks in S phase. Expression of MDC1 is required for the S- and G2/M-checkpoint-mediated cell cycle arrest in response to DNA damage. MDC1 has been shown to interact with H2A.X near sites of DNA double-strand breaks and recruit ATM kinase to the damaged region of DNA. Consistent with this role, MDC1 also appears to modulate the function of other proteins involved in the DNA damage checkpoint such as BRCA1 and Chk2.

Especificidade

This antibody recognizes MDC1 at and around the N-terminus.

Imunogênio

GST-tagged recombinant protein corresponding to mouse MDC1 at and around the N-terminus.

Aplicação

Anti-MDC1 Antibody, clone P2B11 is a Mouse Monoclonal Antibody for detection of MDC1 also known as Nuclear factor with BRCT domains 1 & has been validated in ICC & WB.
Immunocytochemistry Analysis: 1:500 dilution from a previous lot detected MDC1 in NIH/3T3 cells.

Western Blot Analysis: A representative lot was used by an independent laboratory in WB. (Lou, Z., et al. (2003). Nature. 421(6926):957-961.)

Descrição-alvo

~ 184 kDa There are 4 isoforms of human MDC1 because of splicing with molecular weights of approximately 227, 198, 196, and 117 kDa

forma física

Format: Purified
Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) without 0.05% sodium azide.

Outras notas

Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.

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Código de classe de armazenamento

12 - Non Combustible Liquids

Classe de risco de água (WGK)

WGK 1

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Certificados de análise (COA)

Busque Certificados de análise (COA) digitando o Número do Lote do produto. Os números de lote e remessa podem ser encontrados no rótulo de um produto após a palavra “Lot” ou “Batch”.

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