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900622

Sigma-Aldrich

Gelatin methacryloyl

gel strength 300 g Bloom, degree of substitution 60%

Sinônimo(s):

GelMA, Gelatin methacrylamide, Gelatin methacrylate, GelMa, Gelatin Methacrylate

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1 G
R$ 1.344,00

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R$ 1.344,00

About This Item

Fórmula linear:
(C40H59N11O13)n
Código UNSPSC:
12352202
NACRES:
NA.23

R$ 1.344,00

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Nível de qualidade

100

Formulário

powder

temperatura de armazenamento

2-8°C

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Categorias relacionadas

Aplicação

Gelatin methacryloyl can be used to form cross-linked hydrogels for tissue engineering[1] and 3D printing[2][3][4]. It has been used for endothelial cell morphogenesis[5], cardiomyocytes[6], epidermal tissue[7], injectable tissue constructs[8], bone differentiation[9], and cartilage regeneration[10]. Gelatin methacryloyl has been explored in drug delivery applications in the form of microspheres[11] and hydrogels[12].

Código de classe de armazenamento

11 - Combustible Solids

Classe de risco de água (WGK)

WGK 3

Ponto de fulgor (°F)

Not applicable

Ponto de fulgor (°C)

Not applicable

Escolha uma das versões mais recentes:

Certificados de análise (COA)

Lot/Batch Number
MKCT0116
MKCT0113
MKCT0119
MKCS2673
MKCQ6360

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Sílica monodisperse, non-porous, 300 nm

Sigma-Aldrich

904422

Sílica

Gelatina gel strength 80-120 g Bloom, Type A

Sigma-Aldrich

G6144

Gelatina

2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone 98%

Sigma-Aldrich

410896

2-Hydroxy-4′-(2-hydroxyethoxy)-2-methylpropiophenone

Low endotoxin gelatin from porcine skin gel strength 240-360 (Bloom), <10 EU/g Endotoxin

Sigma-Aldrich

901756

Low endotoxin gelatin from porcine skin

Poly(ethylene glycol) diacrylate average Mn 4,000, contains MEHQ as inhibitor

Sigma-Aldrich

907227

Poly(ethylene glycol) diacrylate

Gelatina powder, gel strength ~300 g Bloom, Type A, BioReagent, suitable for electrophoresis, suitable for cell culture

Sigma-Aldrich

G1890

Gelatina

Kristel W M Boere et al.
Acta biomaterialia, 10(6), 2602-2611 (2014-03-05)
Hydrogels can provide a suitable environment for tissue formation by embedded cells, which makes them suitable for applications in regenerative medicine. However, hydrogels possess only limited mechanical strength, and must therefore be reinforced for applications in load-bearing conditions. In most
Xin Zhao et al.
Advanced healthcare materials, 5(1), 108-118 (2015-04-17)
Natural hydrogels are promising scaffolds to engineer epidermis. Currently, natural hydrogels used to support epidermal regeneration are mainly collagen- or gelatin-based, which mimic the natural dermal extracellular matrix but often suffer from insufficient and uncontrollable mechanical and degradation properties. In
Kelly M C Tsang et al.
Advanced functional materials, 25(6), 977-986 (2015-09-04)
Hydrogels are often employed as temporary platforms for cell proliferation and tissue organization in vitro. Researchers have incorporated photodegradable moieties into synthetic polymeric hydrogels as a means of achieving spatiotemporal control over material properties. In this study protein-based photodegradable hydrogels
Anh H Nguyen et al.
Acta biomaterialia, 13, 101-110 (2014-12-03)
Gelatin has been commonly used as a delivery vehicle for various biomolecules for tissue engineering and regenerative medicine applications due to its simple fabrication methods, inherent electrostatic binding properties, and proteolytic degradability. Compared to traditional chemical cross-linking methods, such as
Heemin Kang et al.
Acta biomaterialia, 10(12), 4961-4970 (2014-08-26)
Human induced pluripotent stem cells (hiPSC) are a promising cell source with pluripotency and self-renewal properties. Design of simple and robust biomaterials with an innate ability to induce lineage-specificity of hiPSC is desirable to realize their application in regenerative medicine.
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Engenharia de tecidos

A engenharia de tecidos fabrica culturas de tecidos a partir de estruturas, células vivas e moléculas biologicamente ativas, simulando o microambiente do corpo para reparar ou substituir tecidos danificados.

Tissue Engineering

Tissue engineering fabricates tissues cultures from scaffolds, living cells, and biologically active molecules by simulating the microenvironment of the body to repair or replace damaged tissue.

Questions

1–3 of 3 Questions  

  1. Is this product sterile or not, how to sterilize it?

    1 answer

    1. This product is not sterilized. Sterilization can be achieved by passing a solution through a 0.2 μm PES syringe filter.

      Loessner, D., Meinert, C., Kaemmerer, E. et al. Functionalization, preparation and use of cell-laden gelatin methacryloyl–based hydrogels as modular tissue culture platforms. Nat Protoc 11, 727–746 (2016). https://doi.org/10.1038/nprot.2016.037

      Helpful?

  2. Can you provide guidance on reconstituting the GelMA purchased from Sigma? There is no protocol available online.

    1 answer

    1. There is no protocol available for reconstituting or testing the solubility of the product 900622.
      Here is some information on solubility:
      1.Mix LAP in 3 ml of PBS or cell media at 60°C under stirring to create a 0.5% w/v LAP concentration.
      2.Mix until all LAP is dissolved. Add GelMA to the solution to create a 10% w/v concentration.
      3.Mix at 60°C under stirring until all GelMA is dissolved.

      Helpful?

  3. Sind in dem Produkt Antioxidantien/Inhibitoren wie etwa Topanol A enthalten oder handelt es sich um einen Reinstoff?

    1 answer

    1. The composition of this product is considered proprietary.

      Helpful?

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