HPLC Analysis of Four Proteins on Zenix® SEC-300, Comparison of Column Internal Diameter
Materialien
Analytische Säule
Gelfiltrations-Säule Zenix® SEC-300, 3 μm
L × I.D. 30 cm × 2.1 mm, 3 μm particle size, stainless steelGelfiltrations-Säule Zenix® SEC-300, 3 μm
L × I.D. 30 cm × 4.6 mm, 3 μm particle size, stainless steelGelfiltrations-Säule Zenix® SEC-300, 3 μm
L × I.D. 30 cm × 7.8 mm, 3 μm particle size, stainless steelStandard
CONDITIONS
column
Zenix SEC-300, 30 cm x 2.1 mm I.D., 3 μm particles (Z777024)
column
Zenix SEC-300, 30 cm x 4.6 mm I.D., 3 μm particles (Z777028)
column
Zenix SEC-300, 30 cm x 7.8 mm I.D., 3 μm particles (Z777033)
mobile phase
0.15 M potassium phosphate monobasic, pH 7.0 with phosphoric acid
flow rate
15, 72, 207 μL/min (2.1 mm ID, 4.6 mm ID, 7.8 mm ID, respectively)
pressure
220, 240, 350 psi (2.1 mm ID, 4.6 mm ID, 7.8 mm ID, respectively)
column temp.
25 °C
detector
UV, 215 nm
injection
0.3, 1.4, 4.0 μL (2.1 mm ID, 4.6 mm ID, 7.8 mm ID, respectively)
sample
(Waters SEC200 Protein Std)
Beschreibung
Hinweis zur Analyse
Smaller column internal diameters (ID) are attractive for reasons of low solvent consumption. However, when adequate sample is available, larger ID analytical columns can be advantageous because they provide better column performance.
Rechtliche Hinweise
Zenix is a registered trademark of Sepax Technologies