Direkt zum Inhalt
Merck
  • Determination of hydroxy acids in cosmetics by chemometric experimental design and cyclodextrin-modified capillary electrophoresis.

Determination of hydroxy acids in cosmetics by chemometric experimental design and cyclodextrin-modified capillary electrophoresis.

Electrophoresis (2012-09-22)
Pei-Yu Liu, Yi-Hui Lin, Chia Hsien Feng, Yen-Ling Chen
ZUSAMMENFASSUNG

A CD-modified CE method was established for quantitative determination of seven hydroxy acids in cosmetic products. This method involved chemometric experimental design aspects, including fractional factorial design and central composite design. Chemometric experimental design was used to enhance the method's separation capability and to explore the interactions between parameters. Compared to the traditional investigation that uses multiple parameters, the method that used chemometric experimental design was less time-consuming and lower in cost. In this study, the influences of three experimental variables (phosphate concentration, surfactant concentration, and methanol percentage) on the experimental response were investigated by applying a chromatographic resolution statistic function. The optimized conditions were as follows: a running buffer of 150 mM phosphate solution (pH 7) containing 0.5 mM CTAB, 3 mM γ-CD, and 25% methanol; 20 s sample injection at 0.5 psi; a separation voltage of -15 kV; temperature was set at 25°C; and UV detection at 200 nm. The seven hydroxy acids were well separated in less than 10 min. The LOD (S/N = 3) was 625 nM for both salicylic acid and mandelic acid. The correlation coefficient of the regression curve was greater than 0.998. The RSD and relative error values were all less than 9.21%. After optimization and validation, this simple and rapid analysis method was considered to be established and was successfully applied to several commercial cosmetic products.

MATERIALIEN
Produktnummer
Marke
Produktbeschreibung

Sigma-Aldrich
Natriumphosphat, ACS reagent, ≥99.0%
Sigma-Aldrich
Natriumphosphat Heptahydrat, ACS reagent, 98.0-102.0%
Sigma-Aldrich
Natriumphosphat, puriss. p.a., ACS reagent, anhydrous, ≥99.0% (T)
Sigma-Aldrich
Natriumphosphat dibasisch Dodecahydrat, meets analytical specification of Ph. Eur., BP, E339, 98.5-102.5% (T)
Sigma-Aldrich
Natriumphosphat, 96%
Sigma-Aldrich
Natriumphosphat, BioReagent, for molecular biology, anhydrous, ≥98%
Sigma-Aldrich
Natriumphosphat, puriss., meets analytical specification of Ph. Eur., BP, USP, FCC, E 339, anhydrous, 98-100.5% (calc. to the dried substance)
Sigma-Aldrich
Natriumphosphat, dreibasisch Dodecahydrat, ACS reagent, ≥98%
Sigma-Aldrich
Natriumphosphat, dreibasisch Dodecahydrat, ≥98%
Sigma-Aldrich
Natriumphosphat, for molecular biology, ≥98.5% (titration)
Sigma-Aldrich
Natriumphosphat, zweibasisch -Lösung, BioUltra, 0.5 M in H2O
Sigma-Aldrich
Natriumphosphat, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%
Sigma-Aldrich
Natriumphosphat, BioPerformance Certified, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99.0% (titration)
Sigma-Aldrich
Natriumphosphat, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Natriumphosphat dibasisch Dodecahydrat, BioXtra, ≥99.0% (T)
Sigma-Aldrich
Natriumphosphat monobasisch -Lösung, BioUltra, 5 M in H2O
Sigma-Aldrich
Natriumphosphat, ReagentPlus®, ≥99.0%
Sigma-Aldrich
Natriumphosphat Heptahydrat, ≥99.99% trace metals basis
Sigma-Aldrich
Natriumphosphat, 99.95% trace metals basis
Sigma-Aldrich
Natriumphosphat Heptahydrat, meets USP testing specifications
Sigma-Aldrich
Natriumphosphat, BioXtra, ≥99.0%
Supelco
Phosphatstandard für IC, TraceCERT®, 1000 mg/L phosphate in water (nominal concentration)
Sigma-Aldrich
Natriumphosphat, BioUltra, for molecular biology, ≥99.5% (T)
Sigma-Aldrich
Natriumphosphat, purum p.a., anhydrous, ≥99.0% (T)
Sigma-Aldrich
Natriumphosphat, dreibasisch Dodecahydrat, BioXtra, ≥98.0% (titration)
Sigma-Aldrich
Natriumphosphat, meets USP testing specifications, anhydrous
Sigma-Aldrich
Natriumphosphat, BioXtra, ≥99.0%
Sigma-Aldrich
Natriumphosphat dibasisch Dodecahydrat, tested according to Ph. Eur.
Sigma-Aldrich
Natriumphosphat, purum p.a., anhydrous, ≥98.0% (T)
Sigma-Aldrich
Natriumphosphat, BioReagent, suitable for cell culture, suitable for insect cell culture, ≥99.0%, free-flowing, Redi-Dri