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53311-U
Ascentis® Express Peptide 160 Å ES-C18 (2.7 μm) HPLC Columns
L × I.D. 5 cm × 3 mm, HPLC Column
Synonym(e):
Peptide Separation ES-C18 Column
Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise
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About This Item
UNSPSC-Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
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Produktbezeichnung
Ascentis® Express Peptid ES-C18, 2,7 μm HPLC-Säule, 2.7 μm particle size, L × I.D. 5 cm × 3 mm
Materialien
stainless steel column
Agentur
suitable for USP L1
Produktlinie
Ascentis®
Leistungsmerkmale
endcapped: no
Hersteller/Markenname
Ascentis®
Verpackung
1 ea of
Parameter
≤100 °C temp. range
600 bar max. pressure (9000 psi)
Methode(n)
HPLC: suitable
LC/MS: suitable
UHPLC-MS: suitable
UHPLC: suitable
L × ID
5 cm × 3 mm
Oberflächenbereich
90 m2/g
Verunreinigungen
<5 ppm metals
Matrix
Fused-Core particle platform
superficially porous particle
Aktive Matrixgruppe
C18 (octadecyl) phase
Partikelgröße
2.7 μm
Porengröße
160 Å
pH Betriebsbereich
1-9
Trenntechnik
reversed phase
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Allgemeine Beschreibung
Ascentis Express Peptid ES-C18-Säulen wurden speziell zur Trennung von Verbindungen mit höherer Molekülmasse, wie Peptide und kleine Proteine, entwickelt. Diese Säulen enthalten fortschrittliche Fused-Core-Partikel mit größeren Poren (160 Å im Vergleich zu 90 Å bei Ascentis Express-Standardsäulen), gebunden mit sterisch geschützten C18-Liganden für Extra-Stabilität (ES) bei sehr niedrigem pH-Wert (< 1) und hohen Temperaturen (bis zu 100 ºC). Das erweitert den Anwendungsbereich von Ascentis Express-Säulen beträchtlich.
Rechtliche Hinweise
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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Lagerklassenschlüssel
11 - Combustible Solids
WGK
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
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In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
Meiyun Shi et al.
Journal of separation science, 38(8), 1351-1357 (2015-01-30)
The pentapeptide thymopentin (Arg-Lys-Asp-Val-Tyr, RKDVY) corresponds to amino acids 32-36 of the 49 amino acid immunomodulatory polypeptide, thymopoietin, whose biological activity is partially reproduced. Thymopentin is widely used in the clinic and represents a promising target for drug design but
E Lesellier
Journal of chromatography. A, 1266, 34-42 (2012-11-03)
The recent introduction of new stationary phases for liquid chromatography based on superficially porous particles, called core-shell or fused-core, dramatically improved the separation performances through very high efficiency, due mainly to reduced eddy diffusion. However, few studies have evaluated the
C Gröer et al.
Journal of pharmaceutical and biomedical analysis, 100, 393-401 (2014-09-15)
Cytochrome P450 (CYP) enzymes and UDP-glucuronosyltransferases (UGT) are major determinants in the pharmacokinetics of most drugs on the market. To investigate their impact on intestinal and hepatic drug metabolism, we developed and validated quantification methods for nine CYP (CYP1A2, CYP2B6
Artikel
Choose Ascentis® Express Peptide ES-C18 U/HPLC Columns based on Fused-Core® technology for fast and efficient separation of high-molecular weight compounds, such as peptides and small proteins. With a 2.7 µm particle size and rigorous testing, these columns offer reliable results and high efficiency.
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What is the Department of Transportation shipping information for this product?
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What flow rate should I use with product 53311-U Ascentis® Express Peptide ES C18 HPLC columns?
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Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize product 53311-U Ascentis® Express Peptide ES C18 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
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How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
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The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
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Are guard columns available for product 53311-U Ascentis® Express Peptide ES C18 HPLC Column?
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Yes, we have guard columns available for product 53311-U Ascentis® Express Peptide ES C18 HPLC Column. Product 53500-U is the Ascentis® Express Guard Holder and Product 53537-U Ascentis® Express Peptide ES C18 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 3.0 mm .
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Can I use product 53311-U Ascentis® Express Peptide ES C18 HPLC Column on any type of HPLC system?
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Product 53311-U Ascentis® Express Peptide ES C18 HPLC Column HPLC columns is capable of use on standard HPLC systems as well as UHPLC systems. This column is packed in high pressure hardware capable of withstanding the pressures used in UHPLC systems.
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Can I use product 53311-U Ascentis® Express Peptide ES C18 HPLC Column on a UHPLC system?
1 answer-
Yes. product 53311-U Ascentis® Express Peptide ES C18 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, product 53311-U Ascentis® Express Peptide ES C18 HPLC Column outperforms sub-2 μm micron columns on many applications since product 53311-U Ascentis® Express Peptide ES C18 HPLC Column provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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What is special about product 53311-U Ascentis® Express Peptide ES C18 HPLC Column?
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Product 53311-U Ascentis® Express Peptide ES C18 HPLC Column provides a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis® Express columns provide the benefits of sub-2 μm particles but at much lower backpressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
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Can product 53311-U Ascentis® Express Peptide ES C18 HPLC column be used for LC-MS?
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Yes, Express Fused-Core™ particles were designed with LC-MS in mind. Even extremely short column lengths exhibit sufficient plate counts to show high resolving power. The flat van Deemter plots permit resolution to be maintained at very high flow rates to maximize sample throughput. All Ascentis stationary phases have been evaluated for MS compatibility during their development, and the Express phases are no exception. You can expect extremely low column bleed and background while maintaining longest possible column lifetime. A bonus of product 53311-U Ascentis® Express Peptide ES C18 HPLC Column for high throughput UHPLC and LC-MS is that they are extremely rugged and highly resistant to plugging, a very common failure mode for competitor columns.
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Is there anything special I need to do to my HPLC system to use product 53311-U Ascentis® Express Peptide ES C18 HPLC Column?
1 answer-
Nothing special is required to use product 53311-U Ascentis® Express Peptide ES C18 HPLC Column. To obtain the full benefits of Ascentis® Express Column, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).
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What is the carbon load for product 53311-U Ascentis® Express Peptide ES C18 HPLC Column?
1 answer-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for product 53311-U Ascentis® Express Peptide ES C18 HPLC column is 2 micromoles/meter-squared.
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