Based on the minimum in the van Deemter curves, higher flows than 5um particle columns are required in order to maximize product 53301-U Ascentis® Express Peptide ES C18 HPLC column efficiency. The suggested starting point for flow rate for Ascentis® Express columns: 1.6 mL/min for 4.6 mm ID; 0.8 mL/min for 3.0 mm ID; and 0.4mL/min for 2.1 mm ID.
Fortfahren mit
53301-U
Ascentis® Express Peptid ES-C18, 2,7 μm HPLC-Säule
L × I.D. 5 cm × 2.1 mm, pk of 1 ea
Anmelden zur Ansicht der Organisations- und Vertragspreise.
Größe auswählen
Ansicht ändern
| Größe/SKU | Verfügbarkeit | Preis |
|---|
Über diesen Artikel
UNSPSC Code:
41115700
NACRES:
SB.52
eCl@ss:
32110501
L × i.d.:
5 cm × 2.1 mm
Matrix active group:
C18 (octadecyl) phase
Pore size:
160 Å
Matrix:
Fused-Core particle platform, superficially porous particle
Preise und Verfügbarkeit sind derzeit nicht verfügbar.
Technischer Dienst
Benötigen Sie Hilfe? Unser Team von erfahrenen Wissenschaftlern ist für Sie da.
Unterstützung erhaltenmaterial
stainless steel column
Quality Segment
agency
suitable for USP L1
product line
Ascentis®
mol wt
2000 - 20,000
feature
endcapped: no
packaging
pk of 1 ea
manufacturer/tradename
Ascentis®
parameter
≤100 °C temp. range, 600 bar max. pressure (9000 psi)
technique(s)
HPLC: suitable, LC/MS: suitable, UHPLC-MS: suitable, UHPLC: suitable
L × I.D.
5 cm × 2.1 mm
surface area
90 m2/g
impurities
<5 ppm metals
matrix
Fused-Core particle platform, superficially porous particle
matrix active group
C18 (octadecyl) phase
pore size
160 Å
operating pH range
1-9
separation technique
reversed phase
General description
Ascentis Express Peptid ES-C18-Säulen wurden speziell zur Trennung von Verbindungen mit höherer Molekülmasse, wie Peptide und kleine Proteine, entwickelt. Diese Säulen enthalten fortschrittliche Fused-Core-Partikel mit größeren Poren (160 Å im Vergleich zu 90 Å bei Ascentis Express-Standardsäulen), gebunden mit sterisch geschützten C18-Liganden für Extra-Stabilität (ES) bei sehr niedrigem pH-Wert (< 1) und hohen Temperaturen (bis zu 100 ºC). Das erweitert den Anwendungsbereich von Ascentis Express-Säulen beträchtlich.
Legal Information
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
Still not finding the right product?
Explore all of our products under Ascentis® Express Peptid ES-C18, 2,7 μm HPLC-Säule
Lagerklasse
11 - Combustible Solids
Was ist los?
WGK 3
Flammpunkt (°F)
Not applicable
Flammpunkt (°C)
Not applicable
Hier finden Sie alle aktuellen Versionen:
Besitzen Sie dieses Produkt bereits?
In der Dokumentenbibliothek finden Sie die Dokumentation zu den Produkten, die Sie kürzlich erworben haben.
-
What flow rate should I use with product 53301-U Ascentis® Express Peptide ES C18 HPLC columns?
1 answer-
Helpful?
-
-
What is the pressure rating for product 53301-U Ascentis® Express Peptide ES C18 HPLC Column?
1 answer-
This product 53301-U Ascentis® Express Peptide ES C18 HPLC Column is stable to operating pressures up to 600 bar (9000 psi)
Helpful?
-
-
What is special about product 53301-U Ascentis® Express Peptide ES C18 HPLC Column?
1 answer-
Product 53301-U Ascentis® Express Peptide ES C18 HPLC Column provides a breakthrough in HPLC performance. Based on Fused-Core particle technology, Ascentis® Express columns provide the benefits of sub-2 μm particles but at much lower backpressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
Helpful?
-
-
Do I need special fittings and tubing to connect product 53301-U Ascentis® Express Peptide ES C18 HPLC ColumnExpress columns?
1 answer-
While operating pressures may not exceed the 400 bar (6,000 psi) capability of your traditional instruments, sustained pressures of about 200 bar (3,000 psi) will exceed the recommended pressure for conventional PEEK tubing and fittings at the column inlet. We recommend changing to stainless steel fittings in all high pressure locations and have designed special High Performance HPLC Fittings/Interconnects that will stay tight at pressures of 1,000 bar (15,000 psi) or greater, even when elevated column temperatures are employed.
Helpful?
-
-
What is the carbon load for product 53301-U Ascentis® Express Peptide ES C18 HPLC Column?
1 answer-
Carbon load which is commonly referred to as %Carbon is misleading and not meaningful for Ascentis Express. Since Fused-Core™ particle is unique in the sense that it has a solid core and porous shell, the %Carbon number can not be compared to traditional porous particles. The carbon coverage for product 53301-U Ascentis® Express Peptide ES C18 HPLC column is 2 micromoles/meter-squared.
Helpful?
-
-
Are guard columns available for product 53301-U Ascentis® Express Peptide ES C18 HPLC Column?
1 answer-
Yes, we have guard columns available for product 53301-U Ascentis® Express Peptide ES C18 HPLC Column. Product 53500-U is the Ascentis® Express Guard Holder and Product 53536-U Ascentis® Express Peptide ES C18 Guard Cartridge is a package of three guard cartridges, 2.7 μm particle size, L 5mm × I.D. 2.1 mm .
Helpful?
-
-
What is the Department of Transportation shipping information for this product?
1 answer-
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Helpful?
-
-
How can I measure my instrument bandwidth (IBW) and determine what columns can be used with minimal efficiency loss created by too much internal instrument volume?
1 answer-
The Guide to Dispersion Measurement has simple instructions on how to measure IBW and can be found at sigma-aldrich.com/express.
Helpful?
-
-
Is there anything special I need to do to my HPLC system to use product 53301-U Ascentis® Express Peptide ES C18 HPLC Column?
1 answer-
Nothing special is required to use product 53301-U Ascentis® Express Peptide ES C18 HPLC Column. To obtain the full benefits of Ascentis® Express Column, one should minimize dispersion or instrument bandwidth in the HPLC system (tubing, detector flow cell) as well as confirm the detector response system is set at a fast level. For more information, request Guidelines for Optimizing Performance with Ascentis® Express HPLC Columns (T407102).
Helpful?
-
-
Can I use product 53301-U Ascentis® Express Peptide ES C18 HPLC Column on a UHPLC system?
1 answer-
Yes. product 53301-U Ascentis® Express Peptide ES C18 HPLC columns are packed in a way making them suitable for these ultra high pressure instruments. In fact, product 53301-U Ascentis® Express Peptide ES C18 HPLC Column outperforms sub-2 μm micron columns on many applications since product 53301-U Ascentis® Express Peptide ES C18 HPLC Column provides the benefits of sub-2 μm particles but at much lower back pressure. These benefits include the capability of providing fast HPLC and higher resolution chromatography. The Fused-Core particle consists of a 1.7 μm solid core and a 0.5 μm porous shell. A major benefit of the Fused-Core particle is the small diffusion path (0.5 μm) compared to conventional fully porous particles. The shorter diffusion path reduces axial dispersion of solutes and minimizes peak broadening.
Helpful?
-