U6875

Uridine 5′-triphosphate tris salt

≥90%, P2Y receptor agonist

• Synonym(e): UTP
• Empirische Formel (Hill-System): C₉H₁₅N₂O₁₅P₃
• CAS-Nummer: 108321-53-5
• Molekulargewicht: 484.14
• UNSPSC Code: 12352200
• PubChem Substance ID: 329827645
• NACRES: NA.77
• MDL number: MFCD00058360
• Assay: ≥90%

Eigenschaften

• Produktname: Uridine 5′-triphosphate tris salt, Type VI, ≥90%
• type: Type VI
• Quality Segment: 200
• assay: ≥90%
• solubility: H₂O: soluble 50 mg/mL, clear, colorless
• shipped in: dry ice
• storage temp.: −20°C
• SMILES string: NC(CO)(CO)CO.O[C@H]1[C@@H](O)[C@@H](O[C@@H]1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=CC(=O)NC2=O
• InChI: 1S/C9H15N2O15P3.C4H11NO3/c12-5-1-2-11(9(15)10-5)8-7(14)6(13)4(24-8)3-23-28(19,20)26-29(21,22)25-27(16,17)18;5-4(1-6,2-7)3-8/h1-2,4,6-8,13-14H,3H2,(H,19,20)(H,21,22)(H,10,12,15)(H2,16,17,18);6-8H,1-3,5H2/t4-,6-,7-,8-;/m1./s1
• InChI key: NHMNWWVJZZPKSL-IAIGYFSYSA-N

Beschreibung

Application

Uridine 5′-triphosphate tris salt has been used:

• to investigate its activation potential on the purinergic P2Y2 Receptor in THP1 monocytes
• as a component of non-radioactive nucleoside triphosphate solution used in in vitro RNA polymerase assay
• as a P2Y2 agonist in human embryonic kidney cells (HEK293T) in dynamic mass redistribution (DMR) assays

UTP has been used to study intracellular Ca²⁺ concentration in alveolar type II (AT2) cells. UTP has also been used as a phosphate donor for in vitro kinase assays using deoxycytidine kinase (DCK) or whole cell lysates.

Biochem/physiol Actions

P2Y receptor agonist.

Uridine 5′-triphosphate (UTP) is synthesized from uridine 5′-diphosphate (UDP) in the presence of the enzyme nucleoside diphosphate kinase (NDPK). Plant cells have high uridine as UTP. UTP on amination results in the generation of CTP and is catalysed by enzyme CTP synthetase. UTP acts on the purinergic (P2Y2) receptor and mediates smooth muscle cells (SMCs) mitogenic activation. It also modulates osteopontin expression in SMCs. UTP is a sputum inducer and a promising candidate for assessing the inflammation in respiratory airway.

Preparation Note

Uridine 5′-triphosphate (UTP) tris salt is soluble in water at 50 mg/ml and yields a clear, colorless solution.

Sicherheitshinweise

• Lagerklasse: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable
• ppe: Eyeshields, Faceshields, Gloves, type P2 (EN 143) respirator cartridges