U1875

Urease aus Canavalia ensiformis (Jack bean)

Type III, glycerol solution, 500-800 units/mL

• Synonym(e): Jack bean urease, Urea amidohydrolase
• CAS-Nummer: 9002-13-5
• UNSPSC Code: 12352204
• eCl@ss: 32160410
• NACRES: NA.54
• EG-Nummer: 3.5.1.5 (BRENDA, IUBMB)
• MDL number: MFCD00070858
• Biological source: Canavalia ensiformis
• Concentration: 500-800 units/mL

Eigenschaften

• biological source: Canavalia ensiformis
• Quality Segment: 200
• type: Type III
• form: glycerol solution
• mol wt: ~544620
• concentration: 500-800 units/mL
• storage temp.: 2-8°C

Beschreibung

General description

Molekulargewicht der Untereinheit: ~90,770
Besteht aus sechs Untereinheiten mit einem Molekulargewicht von ~544,620

Urease of jack bean (Canavalia ensiformis), a hexamer of a single chain of 840 amino acid residues, is found in plants, bacteria, fungi, algae, and soil. It is considered the first nickel-containing enzyme and the first enzyme to be crystallized. Ureases exist in several isoforms, some are jack bean urease (JBURE-I), canatoxin (CNTX), and JBURE-IIB. JBURE-I is more abundant than the other two isoforms. Ureases are found in mature seeds.

Application

Urease from Canavalia ensiformis (Jack bean) has been used:

• to assay creatine and creatine phosphate using the sample extracted from the left ventricle (LV) and septal tissue
• to determine the concentration of urea in a stabilized urine sample
• as one of the standards to measure the urease activity of plant extracts

Biochem/physiol Actions

Urease activity has been shown to be inhibited by benzpyrene following exposure to light. This inhibition was due to the effect of benzpyrene on urease′s sulfhydryl groups.

In plants, ureases are essential for the bioavailability/metabolism of nitrogen in nature and defense mechanisms. Ureases are metalloenzymes that catalyze the hydrolysis of urea to release ammonia a nitrogen source for growth and carbon dioxide. They exhibit internal peptides-mediated insecticidal action and fungicidal activity.

Other Notes

One micromolar unit will liberate 1.0 μmole of NH₃ from urea per min at pH 7.0 at 25 °C. It is equivalent to 1.0 I.U. or 0.054 Sumner unit (1.0 mg ammonia nitrogen in 5 minutes at pH 7.0 at 20 °C)

Sicherheitshinweise

• pictograms: GHS08,GHS07
• signalword: Danger
• hcodes: H315,H319,H334,H335
• pcodes: P261 - P264 - P271 - P302 + P352 - P304 + P340 + P312 - P305 + P351 + P338
• Hazard Classifications: Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• target_organs: Respiratory system
• Lagerklasse: 10 - Combustible liquids
• wgk: WGK 1
• flash_point_f: Not applicable
• flash_point_c: Not applicable