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Merck

T7575

Sigma-Aldrich

Trypsin, Singles, Proteomik-Qualität

Synonym(e):

Proteomics grade, Trypsin, TPCK treated

Anmeldenzur Ansicht organisationsspezifischer und vertraglich vereinbarter Preise


About This Item

UNSPSC-Code:
12352204
NACRES:
NA.56

Qualität

Proteomics Grade

Form

ready-to-use solution

Spezifische Aktivität

≥10,000 BAEE units/mg protein

Mol-Gew.

average mol wt 23.29 kDa

Optimaler pH-Wert

~8.0

Lagertemp.

2-8°C

Allgemeine Beschreibung

Experience all of the advantages of Proteomics Grade Trypsin in a convenient, single-use 1 μg package.

Trypsin Singles, Proteomics Grade exhibit excellent proteolytic efficiency, generating more tryptic peptides leading to greater sequence coverage of your peptide of interest. Mass spectra are significantly simplified due to the reduced number of interfering autolytic peaks and their ambiguous adducts. Proteomics Grade Trypsin has been extensively purified from porcine pancreas to enable accurate and precise cleavage on the carboxylic side of Arg and Lys residues. The enzyme has been exhaustively processed by reductive methylation to minimize autolysis and chymotryptic activity has been quenched by TPCK treatment. Further purification steps including affinity chromatography and drying from dilute acid produce a highly purified, high specific activity trypsin purposely suited for the demanding criteria of proteomics research, and designed to function in either solution or gel-based digests.

Anwendung

Trypsin Singles, Proteomics Grade have been used for protein digestion to analyse generated peptides via MALDI-TOF MS (matrix assisted laser desorption ionization-time of flight mass spectrometry). It has been used for the digestion of proteins to analyse generated peptides via LC-MS (liquid chromatography–mass spectrometry)/MS.

Leistungsmerkmale und Vorteile

  • Bequemer Einwegartikel, 1 μg Größe
  • Eliminiert wiederholtes Pipettieren
  • eduktiv methyliert, um autolytische Aktivität zu minimieren
  • TPCK behandelt, um chymotryptische Aktivität zu dämpfen
  • Extensiv gereinigt

Kit-Komponenten auch einzeln erhältlich

Produkt-Nr.
Beschreibung
SDB

  • Trypsin Solubilization Reagent

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Signalwort

Danger

Gefahreneinstufungen

Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Eye Irrit. 2 - Flam. Liq. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3

Zielorgane

Respiratory system

Lagerklassenschlüssel

3 - Flammable liquids


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Die Dokumentenbibliothek aufrufen

Zahraa I Khamis et al.
Journal of Cancer, 1, 70-79 (2010-09-16)
In situ neoplastic prostate cells are not lethal unless they become invasive and metastatic. For cells to become invasive, the prostate gland must undergo degradation of the basement membrane and disruption of the basal cell layer underneath the luminal epithelia.
Tiziana Cabras et al.
Molecular & cellular proteomics : MCP, 9(10), 2099-2108 (2010-06-30)
The acidic soluble fraction of whole saliva of type 1 diabetic children was analyzed by reversed phase (RP)(1)-HPLC-ESI-MS and compared with that of sex- and age-matched control subjects. Salivary acidic proline-rich phosphoproteins (aPRP), histatins, α-defensins, salivary cystatins, statherin, proline-rich peptide
Identification of protein binders in artworks by MALDI-TOF/TOF tandem mass spectrometry.
Tripkovic T, et al.
Talanta, 113, 49-61 (2013)
Melt With This Kiss: Paralyzing and Liquefying Venom of The Assassin Bug Pristhesancus plagipennis (Hemiptera: Reduviidae).
Walker AA, et al.
Molecular and Cellular Proteomics, 16, 552-552 (2017)
David J Gonzalez et al.
The Journal of biological chemistry, 285(36), 28220-28228 (2010-06-29)
Through elaboration of its botulinum toxins, Clostridium botulinum produces clinical syndromes of infant botulism, wound botulism, and other invasive infections. Using comparative genomic analysis, an orphan nine-gene cluster was identified in C. botulinum and the related foodborne pathogen Clostridium sporogenes

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