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SRP4858

Sigma-Aldrich

Heat Shock Protein 90 human

recombinant, expressed in baculovirus infected Sf9 cells, ≥99% (SDS-PAGE)

Synonym(e):

HSP86, HSPC1, HSPCA, HSPN, Hsp89, LAP2

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About This Item

MDL-Nummer:
MFCD02253027
UNSPSC-Code:
12352200
NACRES:
NA.32

Biologische Quelle

human

Rekombinant

expressed in baculovirus infected Sf9 cells

Assay

≥99% (SDS-PAGE)

Form

liquid

Mol-Gew.

~90 kDa

Verpackung

pkg of 25 μg

Lagerbedingungen

avoid repeated freeze/thaw cycles

Verunreinigungen

endotoxin, tested

NCBI-Hinterlegungsnummer

P08238

UniProt-Hinterlegungsnummer

P14625

Versandbedingung

dry ice

Lagertemp.

−70°C

Angaben zum Gen

human ... HSP90B1(7184)

Allgemeine Beschreibung

Heat shock protein 90 (HSP90) is a molecular chaperone and one of the most abundant proteins in unstressed cells. In mammalian cells, there are two genes encoding cytosolic Hsp90 homologues, with the human Hsp90α showing 85% sequence identity to Hsp90β. The recombinant Hsp90 produced from Sf9 cells represents the full length (a.a. 1-723) of Hsp90β subunit.

Biochem./physiol. Wirkung

Heat shock protein 90kDa beta member 1 (HSP90B1) is activated during apoptosis. It is associated with the endoplasmic reticulum membrane and undergoes specific proteolytic cleavage leading to the activation of the caspase CPP32 and initiation of DNA fragmentation. The protein protects cells against flux in Ca2+ homeostasis, which would otherwise result in cell death. It maintains the chromosomal stability in mammalian cells by associating with FAC. HSP90B1 chaperones toll-like receptors (TLRs) and thus optimizes the B cell functioning. These proteins are also the main downstream chaperones which mediate the ER unfolded protein response (UPR), which is very essential for maintaining protein homeostasis in the ER.

Physikalische Form

Protein is provided in a solution of 40 mM HEPES/KOH, pH 7.5, containing 400 mM potassium chloride and 5% glycerol.

Angaben zur Herstellung

Centrifuge the vial prior to opening. Avoid freeze-thaw cycles

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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T Hoshino et al.
Blood, 91(11), 4379-4386 (1998-05-30)
The FAC protein encoded by the gene defective in Fanconi anemia (FA) complementation group C binds to at least three ubiquitous cytoplasmic proteins in vitro. We used here the complete coding sequence of FAC in a yeast two-hybrid screen to
Bei Liu et al.
Blood, 112(4), 1223-1230 (2008-05-30)
Endoplasmic reticulum (ER) unfolded protein response (UPR) plays pivotal roles in both early B-cell development and plasma cell differentiation. As a major ER chaperone to mediate the UPR and a master chaperone for Toll-like receptors (TLRs), HSP90b1 (grp94, gp96) has
R K Reddy et al.
The Journal of biological chemistry, 274(40), 28476-28483 (1999-09-25)
GRP94 is a 94-kDa chaperone glycoprotein with Ca(2+)-binding properties. We report here that during apoptosis induced by the topoisomerase II inhibitor etoposide, a fraction of GRP94 associated with the endoplasmic reticulum membrane undergoes specific proteolytic cleavage, coinciding with the activation

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