SRP0110

HDAC-8 human

recombinant, expressed in baculovirus infected insect cells, ≥80% (SDS-PAGE)

• Synonym(e): CDA07, HD8, HDACL1, Histone deacetylase 8, Histone deacetylase-like 1
• UNSPSC-Code: 12352200
• NACRES: NA.32

Eigenschaften

• Biologische Quelle: human
• Rekombinant: expressed in baculovirus infected insect cells
• Assay: ≥80% (SDS-PAGE)
• Form: aqueous solution
• Wirksamkeit: ≥0.001 units/mg
• Mol-Gew.: 42.4 kDa
• Verpackung: pkg of 50 μg
• Lagerbedingungen: avoid repeated freeze/thaw cycles
• Konzentration: >0.02 mg/mL
• NCBI-Hinterlegungsnummer: NM_018486
• UniProt-Hinterlegungsnummer: Q9BY41
• Versandbedingung: dry ice
• Lagertemp.: −70°C
• Angaben zum Gen: human ... HDAC8(55869)

Beschreibung

Allgemeine Beschreibung

HDAC8 (histone deacetylase 8) is a class I Zn²⁺-dependent SMC3 (structural maintenance of chromosomes 3) deacetylase. It is the human ortholog of the yeast Zn²⁺-dependent deacetylase Hos1.

Anwendung

Useful for the study of enzyme kinetics, screening inhibitors, and selectivity profiling.

Biochem./physiol. Wirkung

HDAC8 (histone deacetylase 8) is a regulator of cohesion protein and is essential for recycling of cohesin during cell cycle. This protein functions on both histone and non-histone substrates, and produces free lysine and acetate by catalyzing the hydrolysis of acetyllysine side chain. Till date, 17 missense mutations in this gene have been identified in patients with Cornelia de Lange Syndrome (CdLS) spectrum disorders, where these mutations have led to partial or complete loss of deacetylase function. This protein is linked with malignant types of neuroblastoma, and its expression in patients with metastatic melanoma is linked with increased survival in the same. Studies show that HDAC8 can abnormally deacetylate and inactivate p53 protein, thus, facilitating leukemia stem cells (LSCs) transformation and maintenance. LSCs are essential for promoting and sustaining acute myeloid leukemia (AML), and HDAC8 inactivation results in normal p53 function in inv(16)(+) AML CD34(+) cells.

Einheitendefinition

One unit is defined as the amount of enzyme required to deacetylate 1 pmol of substrate/min at 37°C.

Physikalische Form

Formulated in 25 mM Tris-HCl, pH 8.0, 138 mM NaCl, 0.05% Tween-20 and 10% glycerol.

Angaben zur Herstellung

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Sicherheitshinweise

• Lagerklassenschlüssel: 12 - Non Combustible Liquids
• WGK: WGK 3
• Flammpunkt (°F): Not applicable
• Flammpunkt (°C): Not applicable