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SHC003

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MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA

Green fluorescent protein marker to monitor transduction efficiency

Synonym(e):

MISSION® Control Vectors

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About This Item

MDL-Nummer:
MFCD07785395
UNSPSC-Code:
41106609
NACRES:
NA.51

Produktlinie

MISSION®

Konzentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

Versandbedingung

dry ice

Lagertemp.

−20°C

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Allgemeine Beschreibung

The MISSION® TurboGFP™ Control Vector is an 8347 base pair lentivirus plasmid vector that contains a gene encoding TurboGFP™, under the control of the CMV promoter. The TurboGFP™ Control Vector is useful as a positive transfection/transduction control in experiments using the MISSION® shRNA library clones. TurboGFP™ is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.

Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The TurboGFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Anwendung

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA has been used to construct GATA2 overexpression vector (oeG2), by removing the GFP cassette of this vector at the 5′ NheI and 3′ PstI cloning sites and replacing it with the full-length GATA2 transcript sequence.
To see more application data, protocols, vector maps visit sigma.com/shrna.

Rechtliche Hinweise

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TurboBeads is a trademark of TurboBeads LLC
TurboGFP is a trademark of Evrogen Co.

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SHC002V

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SHC003V

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SHC004

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SHC014

MISSION® pLKO.1-puro-UbC-TurboGFP™ Positive Control Plasmid DNA, Contains a gene encoding TurboGFP, under the control of the UbC promoter

Lagerklassenschlüssel

12 - Non Combustible Liquids

WGK

WGK 1

Flammpunkt (°F)

Not applicable

Flammpunkt (°C)

Not applicable

Analysenzertifikate (COA)

Suchen Sie nach Analysenzertifikate (COA), indem Sie die Lot-/Chargennummer des Produkts eingeben. Lot- und Chargennummern sind auf dem Produktetikett hinter den Wörtern ‘Lot’ oder ‘Batch’ (Lot oder Charge) zu finden.

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Die Dokumentenbibliothek aufrufen

Selma Zargari et al.
BMC cancer, 20(1), 789-789 (2020-08-21)
MEIS1 (Myeloid ecotropic viral integration site 1) as a homeobox (HOX) transcription factor plays regulatory roles in a variety of cellular processes including development, differentiation, survival, apoptosis and hematopoiesis, as well as stem cell regulation. Few studies have established pluripotency
Inactivation of SDH and FH cause loss of 5hmC and increased H3K9me3 in paraganglioma/pheochromocytoma and smooth muscle tumors.
Hoekstra AS
Oncotarget, 6(36), 38777-38788 (2015)
Tris DBA palladium is highly effective against growth and metastasis of pancreatic cancer in an orthotopic model.
Diaz B
Oncotarget, 7(32), 51569-51580 (2016)
Min Joo Kim et al.
Scientific reports, 9(1), 13288-13288 (2019-09-19)
Although various chemokines have pro-tumorigenic actions in cancers, the effects of CXCL16 remain controversial. The aim of this study was to investigate the molecular characteristics of CXCL16-expressing papillary thyroid cancers (PTCs). CXCL16 expressions were significantly higher in PTCs than benign
Reihaneh Alsadat Mahmoudian et al.
Molecular genetics & genomic medicine, 7(7), e00746-e00746 (2019-05-16)
MEIS1 (Myeloid ecotropic viral integration site 1), as a homeobox (HOX) transcription factor, has a dual function in different types of cancer. Although numerous roles are proposed for MEIS1 in differentiation, stem cell function, gastrointestinal development and tumorigenesis, the involved
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Artikel

shRNA Process and Diagram

When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.

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